TY - JOUR
T1 - Lotus japonicus NF-YA1 plays an essential role during nodule differentiation and targets members of the SHI/STY gene family
AU - Hossain, Md Shakhawat
AU - Shrestha, Arina
AU - Zhong, Sihui
AU - Miri, Mandana
AU - Austin, Ryan S.
AU - Sato, Shusei
AU - Ross, Loretta
AU - Huebert, Terry
AU - Tromas, Alexandre
AU - Torres-Jerez, Ivone
AU - Tang, Yuhong
AU - Udvardi, Michael
AU - Murray, Jeremy D.
AU - Szczyglowski, Krzysztof
N1 - Funding Information:
This work was supported by grants from Agriculture and Agri-Food Canada Crop Genomics Initiative and National Science and Engineering Research Council of Canada (NSERC grant no. 3277A01) to K. Szczyglowski.
Publisher Copyright:
© 2016 The American Phytopathological Society.
PY - 2016/12
Y1 - 2016/12
N2 - Legume plants engage in intimate relationships with rhizobial bacteria to form nitrogen-fixing nodules, root-derived organs that accommodate the microsymbiont.Members of the Nuclear Factor Y (NF-Y) gene family, which have undergone significant expansion and functional diversification during plant evolution, are essential for this symbiotic liaison. Acting in a partially redundant manner, NF-Y proteins were shown, previously, to regulate bacterial infection, including selection of a superior rhizobial strain, and to mediate nodule structure formation. However, the exact mechanism by which these transcriptional factors exert their symbiotic functions has remained elusive. By carrying out detailed functional analyses of Lotus japonicus mutants, we demonstrate that LjNF-YA1 becomes indispensable downstream from the initial cortical cell divisions but prior to nodule differentiation, including cell enlargement and vascular bundle formation. Three affiliates of the SHORT INTERNODES/STYLISH transcription factor gene family, called STY1, STY2, and STY3, are demonstrated to be among likely direct targets of LjNF-YA1, and our results point to their involvement in nodule formation.
AB - Legume plants engage in intimate relationships with rhizobial bacteria to form nitrogen-fixing nodules, root-derived organs that accommodate the microsymbiont.Members of the Nuclear Factor Y (NF-Y) gene family, which have undergone significant expansion and functional diversification during plant evolution, are essential for this symbiotic liaison. Acting in a partially redundant manner, NF-Y proteins were shown, previously, to regulate bacterial infection, including selection of a superior rhizobial strain, and to mediate nodule structure formation. However, the exact mechanism by which these transcriptional factors exert their symbiotic functions has remained elusive. By carrying out detailed functional analyses of Lotus japonicus mutants, we demonstrate that LjNF-YA1 becomes indispensable downstream from the initial cortical cell divisions but prior to nodule differentiation, including cell enlargement and vascular bundle formation. Three affiliates of the SHORT INTERNODES/STYLISH transcription factor gene family, called STY1, STY2, and STY3, are demonstrated to be among likely direct targets of LjNF-YA1, and our results point to their involvement in nodule formation.
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U2 - 10.1094/MPMI-10-16-0206-R
DO - 10.1094/MPMI-10-16-0206-R
M3 - Article
C2 - 27929718
AN - SCOPUS:85010300664
VL - 29
SP - 950
EP - 964
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
SN - 0894-0282
IS - 12
ER -