Lotus japonicus Genetic, Mutant, and Germplasm Resources

Masatsugu Hashiguchi, Hidenori Tanaka, Melody Muguerza, Ryo Akashi, Niels Nørgaard Sandal, Stig Uggerhøj Andersen, Shusei Sato

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

A quarter of a century has passed since Lotus japonicus was proposed as a model legume because of its suitability for molecular genetic studies. Since then, a comprehensive set of genetic resources and tools has been developed, including recombinant inbred lines, a collection of wild accessions, published mutant lines, a large collection of mutant lines tagged with LORE1 insertions, cDNA clones with expressed sequence tag (EST) information, genomic clones with end-sequence information, and a reference genome sequence. Resource centers in Japan and Denmark ensure easy access to data and materials, and the resources have greatly facilitated L. japonicus research, thereby contributing to the molecular understanding of characteristic legume features such as endosymbiosis. Here, we provide detailed instructions for L. japonicus cultivation and describe how to order materials and access data using the resource center websites. The comprehensive overview presented here will make L. japonicus more easily accessible as a model system, especially for research groups new to L. japonicus research.

Original languageEnglish
Pages (from-to)e20070
JournalCurrent protocols in plant biology
Volume3
Issue number2
DOIs
Publication statusPublished - 2018 Jun 1

Keywords

  • LORE1 lines
  • Lotus japonicus
  • genome information browser
  • legume
  • root culture system
  • wild accessions

ASJC Scopus subject areas

  • Medicine(all)

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