Loss of DNA binding ability of the transcription factor encoded by the mutant Mi locus

Eiichi Morii, Kimiko Takebayashi, Hozumi Motohashi, Masayuki Yamamoto, Shintaro Nomura, Yukihiko Kitamura

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The mi locus of mice encodes a novel member of the basic-helix-loop-helix-leucine zipper protein family of transcription factors (MITF). Mutant mice of the mi/mi genotype have the deletion of an arginine in the basic domain and show the abnormality in development of eyes, melanocytes, osteoclasts and mast cells. The expression of the mast cell protease 6 (MMCP-6) gene is markedly reduced in mi/mi mice. We examined the in vitro binding ability of the normal and mutant MITF to a hexameric motif (CACATG) located in the 5′-flanking sequence of the MMCP-6 gene. The normal MITF bound the hexameric motif but the mutant MITF did not, suggesting that the MITF directly regulated the expression of the MMCP-6 gene.

Original languageEnglish
Pages (from-to)1299-1304
Number of pages6
JournalBiochemical and biophysical research communications
Volume205
Issue number2
DOIs
Publication statusPublished - 1994 Dec 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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