Longitudinal diffusion behavior of hemicyanine dyes across phospholipid vesicle membranes as studied by second-harmonic generation and fluorescence spectroscopies

Akira Yamaguchi, Masaki Nakano, Kimihisa Nochi, Tomohisa Yamashita, Kotaro Morita, Norio Teramae

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The adsorption and longitudinal diffusion behaviors of a series of hemicyanine dyes to phospholipid vesicle membranes were studied by second-harmonic generation (SHG) and fluorescence spectroscopies. It was observed that the longitudinal diffusion of cationic hemicyanine dyes takes place immediately after the initial adsorption of these dyes to the outer surface of the vesicle membrane. In contrast, hardly any amount of a zwitterionic hemicyanine dye with a sulfonate group diffused across the vesicle membrane within the measurement time (<2000 s). Based on the difference in the time-course responses of SHG and fluorescence spectroscopies for all of the hemicyanine dyes tested, we propose that hydration of the sulfonate group is mainly responsible for the low diffusivity of the zwitterionic hemicyanine dye.

Original languageEnglish
Pages (from-to)627-632
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume386
Issue number3
DOIs
Publication statusPublished - 2006 Oct 1

Keywords

  • Fluorescence
  • Longitudinal diffusion
  • Phospholipid vesicle
  • Second-harmonic generation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Fingerprint Dive into the research topics of 'Longitudinal diffusion behavior of hemicyanine dyes across phospholipid vesicle membranes as studied by second-harmonic generation and fluorescence spectroscopies'. Together they form a unique fingerprint.

  • Cite this