Long-term potentiation is associated with an increased activity of Ca2+/calmodulin-dependent protein kinase II

K. Fukunaga, L. Stoppini, E. Miyamoto, D. Muller

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342 Citations (Scopus)


Among the molecular mechanisms that have been proposed to contribute to long-term potentiation in hippocampus are the activation and autophosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II). Here we report that high, but not low frequency stimulation applied to two groups of CA1 afferents resulted in a long lasting increase in the Ca2+-independent and total activities of the enzyme as well as an increase in the ratio of Ca2+-independent to total activity. The effect was obtained using two different CaM kinase II substrates, it was observed in hippocampal slices and in hippocampal organotypic cultures, and it could be blocked by preincubation of slices with the N-methyl-D-aspartate receptor antagonist D-2-amino-5-phosphonopentanoate. Treatment of slices with calyculin A, a phosphatase inhibitor, modified the activity of the enzyme, but long term potentiation could still be induced and a further increase in Ca2+-independent CaM kinase II activity still observed.

Original languageEnglish
Pages (from-to)7863-7867
Number of pages5
JournalJournal of Biological Chemistry
Issue number11
Publication statusPublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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