Long-Term Mg2+ Imaging in Live Cells with a Targetable Fluorescent Probe

Priya Ranjan Sahoo, Toshiyuki Kowada, Shin Mizukami

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Living cells dynamically change their morphology and function according to the cell cycle. Long-term observations of living cells are privileged when we spy the unique, cell cycle-driven molecular events, which cannot be obtained from short-term ones. Mg2+, a metal ion abundant in cells, has been shown to be involved in a variety of physiological phenomena by noninvasive cellular observation using fluorescence microscopy. However, long-term observation of Mg2+ in cells has been a great challenge. Herein, we present a protocol for the long-term microscopic imaging of intracellular Mg2+ levels using a small molecule-protein hybrid fluorescent probe we developed.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages237-243
Number of pages7
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2274
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Confocal microscope
  • Fluorescent probe
  • Long-term imaging probe
  • Mg

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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