TY - JOUR
T1 - Long non-coding RNA HOTAIR up-regulates chemokine (C-C motif) ligand 2 and promotes proliferation of macrophages and myeloid-derived suppressor cells in hepatocellular carcinoma cell lines
AU - Fujisaka, Yasuyuki
AU - Iwata, Tomoaki
AU - Tamai, Keiichi
AU - Nakamura, Mao
AU - Mochizuki, Mai
AU - Shibuya, Rie
AU - Yamaguchi, Kazunori
AU - Shimosegawa, Tooru
AU - Satoh, Kennichi
N1 - Funding Information:
This work was supported by JSPS KAKENHI (grant nos. 15K09055 and 16K07132), Japan Agency for Medical Research and Development, and Novartis Pharma Research Grants. This work was partly supported by the Biomedical Research Core of Tohoku University School of Medicine.
Publisher Copyright:
© 2018, Spandidos Publications. All rights reserved.
PY - 2018/1
Y1 - 2018/1
N2 - Accumulating evidence demonstrated that Hox antisense intergenic RNA (HOTAIR) serves essential roles in the development and metastasis of several types of cancer. In hepatocellular carcinoma (HCC), high expression of HOTAIR is associated with poor prognosis, and HOTAIR regulates cell migration and proliferation. However, the downstream molecular targets of HOTAIR depend on the cancer cell types, and little is known about the precise molecular mechanisms of HOTAIR involved in cancer development. The present study investigated the role of HOTAIR in HCC cell lines. Notably, the overexpression of HOTAIR in HCC cell lines did not affect cell migration and proliferation capability. In the microarray analysis, C-C motif chemokine ligand (CCL)2 was identified to be differentially expressed in HOTAIR-overexpressing cells, and it was confirmed that HOTAIR promotes the secretion of CCL2. Furthermore, it was revealed that the proportion of macrophages and myeloid-derived suppressor cells (MDSCs) were increased when peripheral blood mononuclear cells were co-cultured with HOTAIR-overexpressing cells. Collectively, these data suggest that HOTAIR regulates CCL2 expression, which may be involved in the recruitment of macrophages and MDSCs to the tumor microenvironment.
AB - Accumulating evidence demonstrated that Hox antisense intergenic RNA (HOTAIR) serves essential roles in the development and metastasis of several types of cancer. In hepatocellular carcinoma (HCC), high expression of HOTAIR is associated with poor prognosis, and HOTAIR regulates cell migration and proliferation. However, the downstream molecular targets of HOTAIR depend on the cancer cell types, and little is known about the precise molecular mechanisms of HOTAIR involved in cancer development. The present study investigated the role of HOTAIR in HCC cell lines. Notably, the overexpression of HOTAIR in HCC cell lines did not affect cell migration and proliferation capability. In the microarray analysis, C-C motif chemokine ligand (CCL)2 was identified to be differentially expressed in HOTAIR-overexpressing cells, and it was confirmed that HOTAIR promotes the secretion of CCL2. Furthermore, it was revealed that the proportion of macrophages and myeloid-derived suppressor cells (MDSCs) were increased when peripheral blood mononuclear cells were co-cultured with HOTAIR-overexpressing cells. Collectively, these data suggest that HOTAIR regulates CCL2 expression, which may be involved in the recruitment of macrophages and MDSCs to the tumor microenvironment.
KW - CCL2
KW - HOTAIR
KW - Hepatocellular carcinoma
KW - PBMC
KW - Tumor-associated macrophages
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U2 - 10.3892/ol.2017.7322
DO - 10.3892/ol.2017.7322
M3 - Article
AN - SCOPUS:85035345947
VL - 15
SP - 509
EP - 514
JO - Oncology Letters
JF - Oncology Letters
SN - 1792-1074
IS - 1
ER -
