Location of a region of the muscarinic acetylcholine receptor involved in selective effector coupling

Tai Kubo, Hideaki Bujo, Isamu Akiba, Junichi Nakai, Masayoshi Mishina, Shosaku Numa

Research output: Contribution to journalArticlepeer-review

122 Citations (Scopus)

Abstract

Chimaeric muscarinic acetylcholine receptors (mAChR) in which corresponding portions of mAChR I and mAChR II are replaced with each other have been produced in Xenopus oocytes by expression of cDNA constructs encoding them. Functional analysis of the chimaeric mAChRs indicates that a region mostly comprising the putative cytoplasmic portion between the proposed transmembrane segments V and VI is involved in selective coupling of mAChR I and mAChR II with different effector systems. In contrast, the exchange of this region between mAChR I and mAChR II does not significantly affect the antagonist binding properties of the two mAChR subtypes.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalFEBS Letters
Volume241
Issue number1-2
DOIs
Publication statusPublished - 1988 Dec 5
Externally publishedYes

Keywords

  • (Xenopus oocyte)
  • Chimaeric receptor
  • Effector coupling
  • Muscarinic acetylcholine receptor subtype
  • Selective antagonist
  • cDNA expression

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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