Localized electrical stimulation to C2C12 myotubes cultured on a porous membrane-based substrate

Takeshi Ishibashi, Yu Hoshino, Hirokazu Kaji, Makoto Kanzaki, Masaaki Sato, Matsuhiko Nishizawa

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We report a porous membrane-based cell culture device that can conduct localized electrical stimulation of a cell monolayer. The device's cell culture substrate is a microporous alumina membrane with an underlying thin poly(dimethylsiloxane) (PDMS) film spotted with holes. When electric current is generated between the device's Pt ring electrodes-one of which is placed above the cells and the other below the PDMS layer-the current density condenses at the holes in the PDMS film, and cells located above the holes can be electrically stimulated. C2C12 cells were confluently cultured on the substrate and were differentiated to myotubes. To control the stimulated area in the substrate, we attempted to seal and reopen the holes of the PDMS film by using an air bubble. Since the current pulse could be effectively blocked at the sealed holes, fluorescent Ca2+ transients, indicative of cellular excitation, were observed from the myotubes located above holes in the open state.

Original languageEnglish
Pages (from-to)413-419
Number of pages7
JournalBiomedical microdevices
Volume11
Issue number2
DOIs
Publication statusPublished - 2009 Jan 1

Keywords

  • Bubble-sealing
  • C2C12 myotube
  • Catransient
  • Electrical stimulation
  • Porous alumina membrane

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

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