Localization of types I, II and X collagen and osteocalcin in intramembranous, endochondral and chondroid bone of rats

Itaru Mizoguchi, Ichiro Takahashi, Yasuyuki Sasano, Manabu Kagayama, Yoshinori Kuboki, Hideo Mitani

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Chondroid bone is a unique calcified tissue intermediate between bone and cartilage. To clarify its characteristics, we examined the distributions of the ECMs associated with chondrogenic differentiation and matrix calcification in the chondroid bone of the rat glenoid fossa, and compared them to those in two typical bone tissues, alveolar bone of the maxilla (intramembranous bone) and the growth plate of long bone (endochrondral bane), using immunofluorescence techniques. Morphologically, the glenoid fossa consisted of the fibrous, progenitor and cartilaginous cell layers and the cartilaginous cell layer was further divided into the superficial non-hypertrophic layers (secondary cartilage) and the deep hypertrophic cell layers (chondroid bone). The co-distribution of type I and type II collagens was observed in secondary cartilage and chondroid bone, whereas type X collagen was restricted to the pericellular matrix of hypertrophied cells (chondroid bone). Osteo calcin, which was absent from the calcified cartilage of endochondral bone formation, was also present in the ECM of the chondroid bone, but not in cells. These results demonstrate that chondroid bone of rats, which is adjacent to secondary-type cartilage in the glenoid fossa, has phenotypic expressions associated with both hypertrophied chondrocytes and osteocytes.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalAnatomy and Embryology
Volume196
Issue number4
DOIs
Publication statusPublished - 1997 Nov 11

Keywords

  • Bone formation
  • Collagen
  • Extracellular matrix
  • Immunohistochemistry
  • Osteocalcin

ASJC Scopus subject areas

  • Anatomy
  • Embryology
  • Developmental Biology
  • Cell Biology

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