Localization of norepinephrine and serotonin transporter in mouse brain capillary endothelial cells

Kentaro Wakayama, Sumio Ohtsuki, Hitomi Takanaga, Ken Ichi Hosoya, Tetsuya Terasaki

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

Monoamines function as a vasoactive modulator in the central nervous system (CNS) and are believed to regulate blood-brain barrier (BBB) function. Although monoamine transport is an essential process for regulating the extracellular monoamine concentration, the transport systems for monoamines at the BBB are poorly understood. mRNA expression of norepinephrine transporter (NET) and serotonin transporter (SERT) has been detected in a conditionally immortalized mouse brain capillary endothelial cell line (TM-BBB4) used as an in vitro model of the BBB, whereas no dopamine transporter (DAT) was detected. Western blot analysis showed the expression of NET and SERT protein in the membrane fraction of mouse brain capillaries and TM-BBB4 cells. Immunohistochemical analysis revealed that NET and SERT are localized at the brain capillaries in the mouse cerebral cortex, and suggests that NET is localized at the abluminal side of brain capillary endothelial cells, and SERT is localized at the luminal and abluminal sides. NET and SERT expressed at the BBB may be involved in the inactivation of monoamines released from neurons around the BBB.

Original languageEnglish
Pages (from-to)173-180
Number of pages8
JournalNeuroscience Research
Volume44
Issue number2
DOIs
Publication statusPublished - 2002 Oct

Keywords

  • Blood-brain barrier
  • Brain capillary endothelial cell
  • Conditionally immortalized brain capillary endothelial cell line (TM-BBB)
  • Immunohiatochemistry
  • Monoamine
  • Norepinephrine transporter
  • Serotonin transporter

ASJC Scopus subject areas

  • Neuroscience(all)

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