TY - JOUR
T1 - Localization of NAD-isocitrate dehydrogenase and glutamate dehydrogenase in rice roots
T2 - Candidates for providing carbon skeletons to NADH-glutamate synthase
AU - Abiko, Tomomi
AU - Obara, Mitsuhiro
AU - Ushioda, Akiko
AU - Hayakawa, Toshihiko
AU - Hodges, Michael
AU - Yamaya, Tomoyuki
N1 - Funding Information:
We thank Dr K.A. Roubelakis-Angelakis for the kind gift of grapevine GDH antibodies used to confirm the reliability of our maize GDH antibody. We also thank Dr K. Ishiyama for his technical support and valuable suggestions on the immunolocalization work. This work was supported in part by a France–Japan Joint Research Program of JSPS, by Grant-in-aid for Scientific Research (14360035) and that on Priority Area (16085201) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, by Core Research for the Evolutional Science and Technology (CREST) of Japan Science and Technology, and in part by the Project for Rice Genome Research (IP1016) from the Ministry of Agriculture, Forestry and Fisheries of Japan.
PY - 2005/10
Y1 - 2005/10
N2 - In rice roots, transient and cell-type-specific accumulation of both mRNA and protein for NADH-dependent glutamate synthase (NADH-GOGAT) occurs after the supply of NH4+ ions. In order to better understand the origin of 2-oxoglutarate for this reaction, we focused on mitochondrial NAD-dependent isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GDH) in rice roots. Six rice cDNAs encoding a single catalytic (OsIDHa) and two regulatory (OsIDHc;1, OsIDHc;2) IDH subunits and three GDH proteins (OsGDH1-3) were isolated. These genes, except OsGDH3, were expressed in the roots. Real-time PCR analysis showed that OsIDHa and OsIDHc;1 transcripts, but not OsGDH1 and OsGDH2 transcripts, accumulated in a similar manner to NADH-GOGAT mRNA along the crown roots after the supply of different forms of inorganic nitrogen. Furthermore, immunolocalization studies revealed the NH 4+ induction of IDHa protein in two cell layers of the root surface, i.e. epidermis and exodermis, where NADH-GOGAT also accumulated. The possible relationship between NADH-GOGAT, IDH and GDH is discussed. JSPP
AB - In rice roots, transient and cell-type-specific accumulation of both mRNA and protein for NADH-dependent glutamate synthase (NADH-GOGAT) occurs after the supply of NH4+ ions. In order to better understand the origin of 2-oxoglutarate for this reaction, we focused on mitochondrial NAD-dependent isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GDH) in rice roots. Six rice cDNAs encoding a single catalytic (OsIDHa) and two regulatory (OsIDHc;1, OsIDHc;2) IDH subunits and three GDH proteins (OsGDH1-3) were isolated. These genes, except OsGDH3, were expressed in the roots. Real-time PCR analysis showed that OsIDHa and OsIDHc;1 transcripts, but not OsGDH1 and OsGDH2 transcripts, accumulated in a similar manner to NADH-GOGAT mRNA along the crown roots after the supply of different forms of inorganic nitrogen. Furthermore, immunolocalization studies revealed the NH 4+ induction of IDHa protein in two cell layers of the root surface, i.e. epidermis and exodermis, where NADH-GOGAT also accumulated. The possible relationship between NADH-GOGAT, IDH and GDH is discussed. JSPP
KW - 2-Oxoglutarate
KW - Carbon and nitrogen metabolisms
KW - Glutamate dehydrogenase
KW - Isocitrate dehydrogenase
KW - NADH-glutamate synthase
KW - Rice
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U2 - 10.1093/pcp/pci188
DO - 10.1093/pcp/pci188
M3 - Article
C2 - 16120687
AN - SCOPUS:27744532348
VL - 46
SP - 1724
EP - 1734
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 10
ER -