Localization of NAD-isocitrate dehydrogenase and glutamate dehydrogenase in rice roots: Candidates for providing carbon skeletons to NADH-glutamate synthase

Tomomi Abiko, Mitsuhiro Obara, Akiko Ushioda, Toshihiko Hayakawa, Michael Hodges, Tomoyuki Yamaya

    Research output: Contribution to journalArticlepeer-review

    42 Citations (Scopus)

    Abstract

    In rice roots, transient and cell-type-specific accumulation of both mRNA and protein for NADH-dependent glutamate synthase (NADH-GOGAT) occurs after the supply of NH4+ ions. In order to better understand the origin of 2-oxoglutarate for this reaction, we focused on mitochondrial NAD-dependent isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GDH) in rice roots. Six rice cDNAs encoding a single catalytic (OsIDHa) and two regulatory (OsIDHc;1, OsIDHc;2) IDH subunits and three GDH proteins (OsGDH1-3) were isolated. These genes, except OsGDH3, were expressed in the roots. Real-time PCR analysis showed that OsIDHa and OsIDHc;1 transcripts, but not OsGDH1 and OsGDH2 transcripts, accumulated in a similar manner to NADH-GOGAT mRNA along the crown roots after the supply of different forms of inorganic nitrogen. Furthermore, immunolocalization studies revealed the NH 4+ induction of IDHa protein in two cell layers of the root surface, i.e. epidermis and exodermis, where NADH-GOGAT also accumulated. The possible relationship between NADH-GOGAT, IDH and GDH is discussed. JSPP

    Original languageEnglish
    Pages (from-to)1724-1734
    Number of pages11
    JournalPlant and Cell Physiology
    Volume46
    Issue number10
    DOIs
    Publication statusPublished - 2005 Oct

    Keywords

    • 2-Oxoglutarate
    • Carbon and nitrogen metabolisms
    • Glutamate dehydrogenase
    • Isocitrate dehydrogenase
    • NADH-glutamate synthase
    • Rice

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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