Localization of mRNA for fatty acid transport protein in developing and mature brain of rats

Akihiro Utsunomiya, Yuji Owada, Takashi Yoshimoto, Hisatake Kondo

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Gene expression for rat fatty acid transport protein (FATP) and fatty acid translocase (FAT) were examined by Northern and in situ hybridization analysis. In Northern blot analysis of developing brain, FATP mRNA was detected weakly throughout all developing stages without any changes in the expression level, while no gene expression for FAT mRNA was detected at any stages. By in situ hybridization histochemistry, intense expression was seen in the ventricular germinal zone on pre- and perinatal stages, whereas distinct expression was observed in the cerebellar Purkinje and granule cell during postpostnatal development. No expression was detected in the cerebellar external granule cell layer. Because of the high expression of FATP mRNA in the embryonic ventricular zone and the postnatal cerebellar cortical neurons in parallel to the gene expression for fatty acid binding protein (FABP) which we have recently reported, co-operated involvement of FATP and FABP in the active uptake of long-chain fatty acids is plausible in these cells.

Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalMolecular Brain Research
Volume46
Issue number1-2
DOIs
Publication statusPublished - 1997 Jun 1

Keywords

  • Brain
  • Fatty acid translocase
  • Fatty acid transport protein
  • In situ hybridization
  • Ontogeny

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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