TY - JOUR
T1 - Localization of mRNA for fatty acid transport protein in developing and mature brain of rats
AU - Utsunomiya, Akihiro
AU - Owada, Yuji
AU - Yoshimoto, Takashi
AU - Kondo, Hisatake
N1 - Funding Information:
This work was supported by Ministry of Education, Science and Culture of Japan Grant Nos. 07278208, 07457001 and 08270205 (to H.K.) and a grant from Tanaka Orthopedic Clinic Foundation, Hamamatsu, Japan.
PY - 1997/6
Y1 - 1997/6
N2 - Gene expression for rat fatty acid transport protein (FATP) and fatty acid translocase (FAT) were examined by Northern and in situ hybridization analysis. In Northern blot analysis of developing brain, FATP mRNA was detected weakly throughout all developing stages without any changes in the expression level, while no gene expression for FAT mRNA was detected at any stages. By in situ hybridization histochemistry, intense expression was seen in the ventricular germinal zone on pre- and perinatal stages, whereas distinct expression was observed in the cerebellar Purkinje and granule cell during postpostnatal development. No expression was detected in the cerebellar external granule cell layer. Because of the high expression of FATP mRNA in the embryonic ventricular zone and the postnatal cerebellar cortical neurons in parallel to the gene expression for fatty acid binding protein (FABP) which we have recently reported, co-operated involvement of FATP and FABP in the active uptake of long-chain fatty acids is plausible in these cells.
AB - Gene expression for rat fatty acid transport protein (FATP) and fatty acid translocase (FAT) were examined by Northern and in situ hybridization analysis. In Northern blot analysis of developing brain, FATP mRNA was detected weakly throughout all developing stages without any changes in the expression level, while no gene expression for FAT mRNA was detected at any stages. By in situ hybridization histochemistry, intense expression was seen in the ventricular germinal zone on pre- and perinatal stages, whereas distinct expression was observed in the cerebellar Purkinje and granule cell during postpostnatal development. No expression was detected in the cerebellar external granule cell layer. Because of the high expression of FATP mRNA in the embryonic ventricular zone and the postnatal cerebellar cortical neurons in parallel to the gene expression for fatty acid binding protein (FABP) which we have recently reported, co-operated involvement of FATP and FABP in the active uptake of long-chain fatty acids is plausible in these cells.
KW - Brain
KW - Fatty acid translocase
KW - Fatty acid transport protein
KW - In situ hybridization
KW - Ontogeny
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U2 - 10.1016/S0169-328X(96)00303-8
DO - 10.1016/S0169-328X(96)00303-8
M3 - Article
C2 - 9191096
AN - SCOPUS:0343990739
VL - 46
SP - 217
EP - 222
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1-2
ER -