TY - JOUR
T1 - Localization of human β-defensin 3 mRNA in normal oral epithelium, leukoplakia, and lichen planus
T2 - An in situ hybridization study
AU - Nishimura, Michiko
AU - Abiko, Yoshihiro
AU - Kusano, Kaoru
AU - Yamazaki, Mami
AU - Saitoh, Masato
AU - Mizoguchi, Itaru
AU - Jinbu, Yoshinori
AU - Noguchi, Tadahide
AU - Kaku, Tohru
PY - 2003/6/1
Y1 - 2003/6/1
N2 - Human β-defensin 3 (hBD-3), an antimicrobial peptide, is produced by various epithelial and some nonepithelial tissues. hBD-3 mRNA is widely expressed in oral tissues, including oral epithelium and the salivary glands. Although the localization of hBD-1 and hBD-2 has been well demonstrated in tissue sections, the localization pattern of hBD-3 has not yet been shown. In the present study, we investigated the expression pattern of hBD-3 mRNA by in situ hybridization using specific RNA probes; the signal for hBD-3 was detected in upper spinous and granular layers in normal oral epithelium. In cases of leukoplakia, a strong signal of hBD-3 mRNA was observed in the granular layer. In lichen planus, the signal was strongly detected in the spinous and suprabasal layers. The signals were stronger than those of either normal oral epithelium or leukoplakia. The results indicate that the localization pattern of hBD-3 is very similar to that of hBD-2. hBD-2 and hBD-3 may function together or compensate each other for expressional loss.
AB - Human β-defensin 3 (hBD-3), an antimicrobial peptide, is produced by various epithelial and some nonepithelial tissues. hBD-3 mRNA is widely expressed in oral tissues, including oral epithelium and the salivary glands. Although the localization of hBD-1 and hBD-2 has been well demonstrated in tissue sections, the localization pattern of hBD-3 has not yet been shown. In the present study, we investigated the expression pattern of hBD-3 mRNA by in situ hybridization using specific RNA probes; the signal for hBD-3 was detected in upper spinous and granular layers in normal oral epithelium. In cases of leukoplakia, a strong signal of hBD-3 mRNA was observed in the granular layer. In lichen planus, the signal was strongly detected in the spinous and suprabasal layers. The signals were stronger than those of either normal oral epithelium or leukoplakia. The results indicate that the localization pattern of hBD-3 is very similar to that of hBD-2. hBD-2 and hBD-3 may function together or compensate each other for expressional loss.
KW - Antimicrobial peptide
KW - Leukoplakia
KW - Lichen planus
KW - Oral epithelium
KW - β-Defensin 3
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M3 - Article
C2 - 12825122
AN - SCOPUS:0042371615
VL - 36
SP - 94
EP - 97
JO - Medical Electron Microscopy
JF - Medical Electron Microscopy
SN - 0918-4287
IS - 2
ER -