Localization of human β-defensin 3 mRNA in normal oral epithelium, leukoplakia, and lichen planus: An in situ hybridization study

Michiko Nishimura, Yoshihiro Abiko, Kaoru Kusano, Mami Yamazaki, Masato Saitoh, Itaru Mizoguchi, Yoshinori Jinbu, Tadahide Noguchi, Tohru Kaku

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Human β-defensin 3 (hBD-3), an antimicrobial peptide, is produced by various epithelial and some nonepithelial tissues. hBD-3 mRNA is widely expressed in oral tissues, including oral epithelium and the salivary glands. Although the localization of hBD-1 and hBD-2 has been well demonstrated in tissue sections, the localization pattern of hBD-3 has not yet been shown. In the present study, we investigated the expression pattern of hBD-3 mRNA by in situ hybridization using specific RNA probes; the signal for hBD-3 was detected in upper spinous and granular layers in normal oral epithelium. In cases of leukoplakia, a strong signal of hBD-3 mRNA was observed in the granular layer. In lichen planus, the signal was strongly detected in the spinous and suprabasal layers. The signals were stronger than those of either normal oral epithelium or leukoplakia. The results indicate that the localization pattern of hBD-3 is very similar to that of hBD-2. hBD-2 and hBD-3 may function together or compensate each other for expressional loss.

Original languageEnglish
Pages (from-to)94-97
Number of pages4
JournalMedical Electron Microscopy
Volume36
Issue number2
Publication statusPublished - 2003 Jun 1
Externally publishedYes

Keywords

  • Antimicrobial peptide
  • Leukoplakia
  • Lichen planus
  • Oral epithelium
  • β-Defensin 3

ASJC Scopus subject areas

  • Anatomy

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