Localization of brain-type fatty acid-binding protein in Kupffer cells of mice and its transient decrease in response to lipopolysaccharide

Soha Abdelkawi Abdelwahab, Yuji Owada, Noriko Kitanaka, Hiroo Iwasa, Hiroyuki Sakagami, Hisatake Kondo

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Brain-type fatty acid-binding protein (B-FABP) was localized in Kupffer cells of liver of postnatal day 10 (P10) and older mice in immunolight and electron microscopy as well as by in situ hybridization histochemistry. The immunoreaction products were localized in the cytoplasmic matrix but not within the nucleus. After peritoneal injection of lipopolysaccharide (LPS), the immunoreaction for B-FABP decreased markedly in Kupffer cells at 1 h postinjection and thereafter gradually recovered to the preinjection level by 24 h postinjection, although no decrease in the mRNA expression was detected in Northern blotting throughout the course after the injection. The specific localization of B-FABP, but not the other FABPs, in Kupffer cells, and its rapid decrease after LPS injection suggest the intimate involvement of B-FABP in Kupffer cells in the inflammatory reaction, probably through mediation of n-3 polyunsaturated fatty acids, which are strong binders of B-FABP.

Original languageEnglish
Pages (from-to)469-475
Number of pages7
JournalHistochemistry and Cell Biology
Volume119
Issue number6
DOIs
Publication statusPublished - 2003 Jun 1

Keywords

  • Brain
  • Fatty acid binding protein
  • Kupffer cell
  • Lipopolysaccharide
  • Liver

ASJC Scopus subject areas

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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