Localization of acid phosphatase activities in the roots of white lupin plants grown under phosphorus-deficient conditions

Jun Wasaki, Soichi Kojima, Hayato Maruyama, Susan Haase, Mitsuru Osaki, Ellen Kandeler

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Acid phosphatase (APase) produced by the cluster roots of white lupin (Lupinus albus L.) plays an important role in inorganic phosphate (Pi) acquisition. Although the importance of cluster roots in Pi acquisition is well known, information on the distribution of APase within tissues of normal and cluster roots is lacking. Isoelectric focusing of APase isoforms as well as histochemical localization and visualization of APase were used to clarify the importance of secretory APase for P nutrition of white lupin grown under P deficiency. Isoelectric focusing revealed that both the secretory type and other major APase isoforms probably involved in P translocation were inducible. The major activity in the rhizosphere soil of cluster roots and roots grown under hydroponic conditions corresponded to LASAP2, a previously purified APase secreted from white lupin roots. Histochemical localization using enzyme-labeled fluorescence (ELF)-97 phosphate as a substrate was applied to rhizosphere samples. This substrate provides fluorescent precipitates after hydrolysis by phosphatase. Strong APase activity in the epidermal tissues of normal roots and cluster rootlets and in root hairs of cluster rootlets under P deficiency was detected. These results support the hypothesis that APase activities in the rhizosphere liberate Pi and supply it to white lupin plants grown under P-deficient conditions.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalSoil Science and Plant Nutrition
Volume54
Issue number1
DOIs
Publication statusPublished - 2008 Feb 1
Externally publishedYes

Keywords

  • Acid phosphatase
  • Cluster roots
  • ELF-97 phosphate
  • Lupinus albus
  • Phosphorus deficiency

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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