Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation

Seunghoon Lee, Yuki Hiradate, Yumi Hoshino, Yeoung Gyu Ko, Kentaro Tanemura, Eimei Sato

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Many studies of the main gap junction protein, Cx43, have been conducted in porcine oocyte research, but they have been limited to investigations of cumulus-oocyte complexes (COCs). In this study, we verified Cx43 not in COCs, but in porcine oocytes during maturation, and conducted a quantitative time course analysis. The location and dynamics of Cx43 were examined by immunocytochemistry and western blotting, respectively. COCs were cultured in NCSU23 medium and processed for immunocytochemistry and western blotting at 0, 14, 28, and 42 h after denuding. A Cx43 signal was detected on oolemmas, transzonal projections and the surface of zona pellucidae. Western blotting showed that Cx43 band density increased from 0 to 14 h, and gradually decreased thereafter. Our results clarified that Cx43 is localized in the ooplasmic membrane through zona pellucidae and its level changes over time during culture in porcine oocytes.

Original languageEnglish
Pages (from-to)364-370
Number of pages7
JournalZygote
Volume24
Issue number3
DOIs
Publication statusPublished - 2016 Jun 1

Keywords

  • Connexin43
  • Localization
  • Porcine oocyte
  • Quantitative analysis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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