TY - JOUR
T1 - Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation
AU - Lee, Seunghoon
AU - Hiradate, Yuuki
AU - Hoshino, Yumi
AU - Ko, Yeoung Gyu
AU - Tanemura, Kentaro
AU - Sato, Eimei
N1 - Funding Information:
This work was supported by a grant from the Japan Society for the Promotion of Science to E. Sato (no. 21248032) and a fund of Project No. PJ009321
Publisher Copyright:
© CopyrightCambridge University Press 2015.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Many studies of the main gap junction protein, Cx43, have been conducted in porcine oocyte research, but they have been limited to investigations of cumulus-oocyte complexes (COCs). In this study, we verified Cx43 not in COCs, but in porcine oocytes during maturation, and conducted a quantitative time course analysis. The location and dynamics of Cx43 were examined by immunocytochemistry and western blotting, respectively. COCs were cultured in NCSU23 medium and processed for immunocytochemistry and western blotting at 0, 14, 28, and 42 h after denuding. A Cx43 signal was detected on oolemmas, transzonal projections and the surface of zona pellucidae. Western blotting showed that Cx43 band density increased from 0 to 14 h, and gradually decreased thereafter. Our results clarified that Cx43 is localized in the ooplasmic membrane through zona pellucidae and its level changes over time during culture in porcine oocytes.
AB - Many studies of the main gap junction protein, Cx43, have been conducted in porcine oocyte research, but they have been limited to investigations of cumulus-oocyte complexes (COCs). In this study, we verified Cx43 not in COCs, but in porcine oocytes during maturation, and conducted a quantitative time course analysis. The location and dynamics of Cx43 were examined by immunocytochemistry and western blotting, respectively. COCs were cultured in NCSU23 medium and processed for immunocytochemistry and western blotting at 0, 14, 28, and 42 h after denuding. A Cx43 signal was detected on oolemmas, transzonal projections and the surface of zona pellucidae. Western blotting showed that Cx43 band density increased from 0 to 14 h, and gradually decreased thereafter. Our results clarified that Cx43 is localized in the ooplasmic membrane through zona pellucidae and its level changes over time during culture in porcine oocytes.
KW - Connexin43
KW - Localization
KW - Porcine oocyte
KW - Quantitative analysis
UR - http://www.scopus.com/inward/record.url?scp=84968820385&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84968820385&partnerID=8YFLogxK
U2 - 10.1017/S0967199415000271
DO - 10.1017/S0967199415000271
M3 - Article
C2 - 27172054
AN - SCOPUS:84968820385
VL - 24
SP - 364
EP - 370
JO - Zygote
JF - Zygote
SN - 0967-1994
IS - 3
ER -