Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation

Seunghoon Lee, Yuuki Hiradate, Yumi Hoshino, Yeoung Gyu Ko, Kentaro Tanemura, Eimei Sato

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Many studies of the main gap junction protein, Cx43, have been conducted in porcine oocyte research, but they have been limited to investigations of cumulus-oocyte complexes (COCs). In this study, we verified Cx43 not in COCs, but in porcine oocytes during maturation, and conducted a quantitative time course analysis. The location and dynamics of Cx43 were examined by immunocytochemistry and western blotting, respectively. COCs were cultured in NCSU23 medium and processed for immunocytochemistry and western blotting at 0, 14, 28, and 42 h after denuding. A Cx43 signal was detected on oolemmas, transzonal projections and the surface of zona pellucidae. Western blotting showed that Cx43 band density increased from 0 to 14 h, and gradually decreased thereafter. Our results clarified that Cx43 is localized in the ooplasmic membrane through zona pellucidae and its level changes over time during culture in porcine oocytes.

    Original languageEnglish
    Pages (from-to)364-370
    Number of pages7
    JournalZygote
    Volume24
    Issue number3
    DOIs
    Publication statusPublished - 2016 Jun 1

    Keywords

    • Connexin43
    • Localization
    • Porcine oocyte
    • Quantitative analysis

    ASJC Scopus subject areas

    • Developmental Biology
    • Cell Biology

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