TY - JOUR
T1 - Localization and osteoblastic differentiation potential of neural crest-derived cells in oral tissues of adult mice
AU - Ono, Miki
AU - Suzawa, Tetsuo
AU - Takami, Masamichi
AU - Yamamoto, Gou
AU - Hosono, Tomohiko
AU - Yamada, Atsushi
AU - Suzuki, Dai
AU - Yoshimura, Kentaro
AU - Watahiki, Junichi
AU - Hayashi, Ryuhei
AU - Arata, Satoru
AU - Mishima, Kenji
AU - Nishida, Kohji
AU - Osumi, Noriko
AU - Maki, Koutaro
AU - Kamijo, Ryutaro
N1 - Funding Information:
We are grateful to Ayumi Ogasawara, Tohoku University Graduate School of Medicine, for the technical assistance. We thank all of the members of the Department of Biochemistry, School of Dentistry, Showa University, for their valuable discussion. This work was supported in part by The Project to Establish Strategic Research Center for Innovative Dentistry by the Ministry of Education, Culture, Sports, Science and Technology of Japan, 2010–2015, and by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science .
Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.
PY - 2015/9/4
Y1 - 2015/9/4
N2 - In embryos, neural crest cells emerge from the dorsal region of the fusing neural tube and migrate throughout tissues to differentiate into various types of cells including osteoblasts. In adults, subsets of neural crest-derived cells (NCDCs) reside as stem cells and are considered to be useful cell sources for regenerative medicine strategies. Numerous studies have suggested that stem cells with a neural crest origin persist into adulthood, especially those within the mammalian craniofacial compartment. However, their distribution as well as capacity to differentiate into osteoblasts in adults is not fully understood. To analyze the precise distribution and characteristics of NCDCs in adult oral tissues, we utilized an established line of double transgenic (P0-Cre/CAG-CAT-EGFP) mice in which NCDCs express green fluorescent protein (GFP) throughout their life. GFP-positive cells were scattered like islands throughout tissues of the palate, gingiva, tongue, and buccal mucosa in adult mice, with those isolated from the latter shown to form spheres, typical cell clusters composed of stem cells, under low-adherent conditions. Furthermore, GFP-positive cells had markedly increased alkaline phosphatase (a marker enzyme of osteoblast differentiation) activity and mineralization as shown by alizarin red staining, in the presence of bone morphogenetic protein (BMP)-2. These results suggest that NCDCs reside in various adult oral tissues and possess potential to differentiate into osteoblastic cells. NCDCs in adults may be a useful cell source for bone regeneration strategies.
AB - In embryos, neural crest cells emerge from the dorsal region of the fusing neural tube and migrate throughout tissues to differentiate into various types of cells including osteoblasts. In adults, subsets of neural crest-derived cells (NCDCs) reside as stem cells and are considered to be useful cell sources for regenerative medicine strategies. Numerous studies have suggested that stem cells with a neural crest origin persist into adulthood, especially those within the mammalian craniofacial compartment. However, their distribution as well as capacity to differentiate into osteoblasts in adults is not fully understood. To analyze the precise distribution and characteristics of NCDCs in adult oral tissues, we utilized an established line of double transgenic (P0-Cre/CAG-CAT-EGFP) mice in which NCDCs express green fluorescent protein (GFP) throughout their life. GFP-positive cells were scattered like islands throughout tissues of the palate, gingiva, tongue, and buccal mucosa in adult mice, with those isolated from the latter shown to form spheres, typical cell clusters composed of stem cells, under low-adherent conditions. Furthermore, GFP-positive cells had markedly increased alkaline phosphatase (a marker enzyme of osteoblast differentiation) activity and mineralization as shown by alizarin red staining, in the presence of bone morphogenetic protein (BMP)-2. These results suggest that NCDCs reside in various adult oral tissues and possess potential to differentiate into osteoblastic cells. NCDCs in adults may be a useful cell source for bone regeneration strategies.
KW - Adult
KW - Cellular differentiation
KW - Neural crest-derived cells
KW - Oral tissues
KW - Osteoblasts
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U2 - 10.1016/j.bbrc.2015.07.106
DO - 10.1016/j.bbrc.2015.07.106
M3 - Article
C2 - 26225748
AN - SCOPUS:84940461221
VL - 464
SP - 1209
EP - 1214
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -