LjABCB1, an ATP-binding cassette protein specifically induced in uninfected cells of Lotus japonicus nodules

Kojiro Takanashi, Akifumi Sugiyama, Shusei Sato, Satoshi Tabata, Kazufumi Yazaki

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Legume plants develop root nodules through symbiosis with rhizobia, and fix atmospheric nitrogen in this symbiotic organ. Development of root nodules is regulated by many metabolites including phytohormones. Previously, we reported that auxin is strongly involved in the development of the nodule vascular bundle and lenticel formation on the nodules of Lotus japonicus. Here we show that an ATP-binding cassette (ABC) protein, LjABCB1, which is a homologue of Arabidopsis auxin transporter AtABCB4, is specifically expressed during nodulation of L. japonicus. A reporter gene analysis indicated that the expression of LjABCB1 was restricted to uninfected cells adjacent to infected cells in the nodule, while no expression was observed in shoot apical meristems or root tips, in which most auxin transporter genes are expressed. The auxin transport activity of LjABCB1 was confirmed using a heterologous expression system.

Original languageEnglish
Pages (from-to)322-326
Number of pages5
JournalJournal of Plant Physiology
Volume169
Issue number3
DOIs
Publication statusPublished - 2012 Feb 15
Externally publishedYes

Keywords

  • ABC protein
  • Auxin
  • Lotus japonicus
  • Nodule
  • Symbiotic nitrogen fixation

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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