Live staining and isolation of specific hormone-producing cells from rat anterior pituitary by cytochemistry with lectins and cholera toxin B subunit

Motoshi Kikuchi, Kenji Kusumoto, Ken Fujiwara, Kozue Takahashi, Yukiko Tando, Takashi Yashiro

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Anterior ptuitay glandsf constan five types of hormone-producing cells. Distinguishing and isolating specific types of living cells are essential for studying their function. Although many such attempts have been made, the results have been disappointing. In the present study, we labeled specific types of living hormone-producing cells by using potential differences in sugar chains on the cell surfaces. Cytochemical analysis with lectins and cholera toxin B subunit revealed that PNA, S-WGA, and cholera toxin B subunit recognized sugar chains specific to prolactin cells, ACTH cells, and GH cells, respectively, and that UEA-I recognized most of prolactin cells and GH cells. Next, fluorescence-activated cell sorting was used to isolate GH cells labeled by fluoresceinated cholera toxin B. The purity of the GH cell fraction estimated by immunocytochemistry and quantitative real-time PCR for cell type-specific genes was more than 98%, which was higher than that reported in earlier studies, including those using transgenic animals. We conclude that cytochemistry with lectins and cholera toxin B subunit is a straightforward, acceptable method of isolating specific types of anterior pituitary cells and that the cells isolated by this method can serve as useful materials in the study of anterior pituitary cells.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalACTA HISTOCHEMICA ET CYTOCHEMICA
Volume44
Issue number4
DOIs
Publication statusPublished - 2011

Keywords

  • Anterior pituitary
  • Cell sorting
  • Cholera toxin
  • Cytochemistry
  • Lectin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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