Live-cell imaging of zygotic intracellular structures and early embryo pattern formation in Arabidopsis thaliana

Minako Ueda, Yusuke Kimata, Daisuke Kurihara

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Plant embryogenesis begins with fertilization and ends with the generation of the basic body plan of the future plant. Despite its importance, the dynamics of flowering plant ontogeny have long been a mystery, because the embryo develops deep in the maternal tissue. Recently, an embryonic live-cell imaging system was established in Arabidopsis thaliana by developing an in vitro ovule cultivation method and utilizing two-photon excitation microscopy (2PEM), which is suitable for deep imaging. This system enabled us to visualize intracellular dynamics during zygote polarization and monitor the cell division pattern during embryogenesis from the zygote until organ formation. In this chapter, we describe a method that allows for high-resolution imaging of cytoskeletal rearrangements in the zygote and long-term tracing of embryo patterning.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages37-47
Number of pages11
DOIs
Publication statusPublished - 2020
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2122
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Arabidopsis thaliana
  • Embryo
  • Live-cell imaging
  • Two-photon excitation microscopy
  • Zygote

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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