Live Cell imaging of endogenous mRNA Using RNA-Based fluorescence "turn-on" probe

Wei Qiang Ong, Y. Rose Citron, Sayaka Sekine, Bo Huang

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.

Original languageEnglish
Pages (from-to)200-205
Number of pages6
JournalACS Chemical Biology
Issue number1
Publication statusPublished - 2017 Jan 20
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine


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