Live-Cell Imaging and Optical Manipulation of Arabidopsis Early Embryogenesis

Keita Gooh, Minako Ueda, Kana Aruga, Jongho Park, Hideyuki Arata, Tetsuya Higashiyama, Daisuke Kurihara

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

Intercellular communications are essential for cell proliferation and differentiation during plant embryogenesis. However, analysis of intercellular communications in living material in real time is difficult owing to the restricted accessibility of the embryo within the flower. We established a live-embryo imaging system to visualize cell division and cell fate specification in Arabidopsis thaliana from zygote division in real time. We generated a cell-division lineage tree for early embryogenesis in Arabidopsis. Lineage analysis showed that both the direction and time course of cell division between sister cells differed along the apical-basal or radial axes. Using the Arabidopsis kpl mutant, in which single-fertilization events are frequent, we showed that endosperm development is not required for pattern formation during early embryogenesis. Optical manipulation demonstrated that damage to the embryo initial cell induces cell fate conversion of the suspensor cell to compensate for the disrupted embryo initial cell even after cell fate is specified. Gooh et al. establish a live-embryo imaging system for Arabidopsis and generate a complete lineage tree from double fertilization in early embryogenesis. They provide a platform for real-time analysis of cell division dynamics and cell fate specification using optical manipulation and micro-engineering techniques such as laser irradiation of specific cells.

Original languageEnglish
Pages (from-to)242-251
Number of pages10
JournalDevelopmental cell
Volume34
Issue number2
DOIs
Publication statusPublished - 2015 Jul 27
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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