Lipoteichoic acid downregulates Fcε{lunate}RI expression on human mast cells through Toll-like receptor 2

Mino Yoshioka, Nobuyuki Fukuishi, Sayuri Iriguchi, Kanae Ohsaki, Hiroyuki Yamanobe, Asumi Inukai, Daisuke Kurihara, Naoki Imajo, Yumiko Yasui, Nobuaki Matsui, Tadayuki Tsujita, Akihiro Ishii, Tsukasa Seya, Makoto Takahama, Masaaki Akagi

Research output: Contribution to journalArticlepeer-review

66 Citations (Scopus)

Abstract

Background: Fcε{lunate}RI on the surface of mast cells (MCs) plays a central role in allergic responses. Recent evidence shows that exposure to microbial components corresponds with a significant reduction in the risk for allergic diseases. Although many reports suggest that this is due to changes in T-cell functions, how MC functions are altered by bacterial infection remains unknown. Objective: We sought to elucidate the effect of bacterial infection on MC function and expression of Fc receptors, such as Fcε{lunate}RI. Methods: Isolated human pulmonary MCs and a human MC line (LAD2) were stimulated with bacterial components, and the function and surface expression of Fc receptors were measured. Results: Lipoteichoic acid (LTA) and peptidoglycan, but not LPS, flagellin, or 3CpG-oligodeoxynucleotide, reduced the expression of Fcε{lunate}RI on LAD2 cells. An antibody to Toll-like receptor (TLR) 2 partially blocked the effect of LTA but not peptidoglycan. Both LTA and peptidoglycan reduced MC degranulation caused by an antigen-specific IgE. Furthermore, exposure of pulmonary MCs to LTA reduced both Fcε{lunate}RI expression and IgE-induced degranulation. None of the bacterial components affected the expression of other Fc receptors, such as Fcγ receptors or Fcα receptor I. Conclusions: Our results indicate that LTA reduces the surface expression of Fcε{lunate}RI through TLR2 and suggests that TLR2 ligands could be used as a novel therapy for controlling allergic disorders. Clinical implications: By knowing how bacterial components modulate MC function, we can expand our possibilities for therapeutic interventions of allergic diseases.

Original languageEnglish
Pages (from-to)452-461
Number of pages10
JournalJournal of Allergy and Clinical Immunology
Volume120
Issue number2
DOIs
Publication statusPublished - 2007 Aug

Keywords

  • Fc receptors
  • Mast cells
  • allergy
  • basophils

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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