Lipocalin-type prostaglandin D synthase as a melanocyte marker regulated by MITF

Kazuhisa Takeda, Satoru Yokoyama, Hiroyuki Aburatani, Takayuki Masuda, Feng Han, Miki Yoshizawa, Naomi Yamaki, Hiroaki Yamamoto, Naomi Eguchi, Yoshihiro Urade, Shigeki Shibahara

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Microphthalmia-associated transcription factor (MITF) is responsible for differentiation of melanocytes. A recessive MITF mutant, black-eyed white Mitfmi-bw mouse, is characterized by white coat color and deafness, due to the lack of melanocytes in the skin and inner ears. By cDNA microarray analysis, we have identified lipocalin-type prostaglandin D synthase (L-PGDS), whose mRNA is undetectable in the homozygous Mitfmi-bw skin. Immunohistochemical analysis of wild-type mice identified the specific expression of L-PGDS in follicular melanocytes. L-PGDS mRNA is expressed in B16 mouse melanoma cells, but undetectable in human melanoma cell lines. RNA interference analysis against MITF suggests that L-PGDS expression is dependent on MITF in B16 melanoma cells. Furthermore, we have provided evidence that MITF is involved in the melanocyte lineage-specific transcription of the mouse L-PGDS gene. Thus, L-PGDS represents a newly identified melanocyte marker. MITF may modulate the production of prostaglandin D2 by activating the L-PGDS gene in melanocytes.

Original languageEnglish
Pages (from-to)1098-1106
Number of pages9
JournalBiochemical and biophysical research communications
Volume339
Issue number4
DOIs
Publication statusPublished - 2006 Jan 27

Keywords

  • Lipocalin-type prostaglandin D synthase
  • Melanocyte
  • Melanoma
  • Microphthalmia-associated transcription factor
  • Prostaglandin D
  • Skin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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