TY - JOUR
T1 - Lipocalin-type prostaglandin D synthase as a melanocyte marker regulated by MITF
AU - Takeda, Kazuhisa
AU - Yokoyama, Satoru
AU - Aburatani, Hiroyuki
AU - Masuda, Takayuki
AU - Han, Feng
AU - Yoshizawa, Miki
AU - Yamaki, Naomi
AU - Yamamoto, Hiroaki
AU - Eguchi, Naomi
AU - Urade, Yoshihiro
AU - Shibahara, Shigeki
N1 - Funding Information:
We thank Prof. Tetsuo Noda for helpful discussion concerning the microarray analysis. This study was supported by Grants-in-aid for Scientific Research (B), for Scientific Research on Priority Areas, for Scientific Research (C) (to K.T.), and by the 21st Century COE Program Special Research Grant “The Center for Innovative Therapeutic Development for Common Diseases” from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2006/1/27
Y1 - 2006/1/27
N2 - Microphthalmia-associated transcription factor (MITF) is responsible for differentiation of melanocytes. A recessive MITF mutant, black-eyed white Mitfmi-bw mouse, is characterized by white coat color and deafness, due to the lack of melanocytes in the skin and inner ears. By cDNA microarray analysis, we have identified lipocalin-type prostaglandin D synthase (L-PGDS), whose mRNA is undetectable in the homozygous Mitfmi-bw skin. Immunohistochemical analysis of wild-type mice identified the specific expression of L-PGDS in follicular melanocytes. L-PGDS mRNA is expressed in B16 mouse melanoma cells, but undetectable in human melanoma cell lines. RNA interference analysis against MITF suggests that L-PGDS expression is dependent on MITF in B16 melanoma cells. Furthermore, we have provided evidence that MITF is involved in the melanocyte lineage-specific transcription of the mouse L-PGDS gene. Thus, L-PGDS represents a newly identified melanocyte marker. MITF may modulate the production of prostaglandin D2 by activating the L-PGDS gene in melanocytes.
AB - Microphthalmia-associated transcription factor (MITF) is responsible for differentiation of melanocytes. A recessive MITF mutant, black-eyed white Mitfmi-bw mouse, is characterized by white coat color and deafness, due to the lack of melanocytes in the skin and inner ears. By cDNA microarray analysis, we have identified lipocalin-type prostaglandin D synthase (L-PGDS), whose mRNA is undetectable in the homozygous Mitfmi-bw skin. Immunohistochemical analysis of wild-type mice identified the specific expression of L-PGDS in follicular melanocytes. L-PGDS mRNA is expressed in B16 mouse melanoma cells, but undetectable in human melanoma cell lines. RNA interference analysis against MITF suggests that L-PGDS expression is dependent on MITF in B16 melanoma cells. Furthermore, we have provided evidence that MITF is involved in the melanocyte lineage-specific transcription of the mouse L-PGDS gene. Thus, L-PGDS represents a newly identified melanocyte marker. MITF may modulate the production of prostaglandin D2 by activating the L-PGDS gene in melanocytes.
KW - Lipocalin-type prostaglandin D synthase
KW - Melanocyte
KW - Melanoma
KW - Microphthalmia-associated transcription factor
KW - Prostaglandin D
KW - Skin
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U2 - 10.1016/j.bbrc.2005.11.125
DO - 10.1016/j.bbrc.2005.11.125
M3 - Article
C2 - 16337607
AN - SCOPUS:29044445875
VL - 339
SP - 1098
EP - 1106
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -