TY - JOUR
T1 - Lipocalin 2 regulation by thermal stresses
T2 - Protective role of Lcn2/NGAL against cold and heat stresses
AU - Roudkenar, Mehryar Habibi
AU - Halabian, Raheleh
AU - Roushandeh, Amaneh Mohammadi
AU - Nourani, Mohammad Reza
AU - Masroori, Nasser
AU - Ebrahimi, Majid
AU - Nikogoftar, Mahin
AU - Rouhbakhsh, Mehdi
AU - Bahmani, Parisa
AU - Najafabadi, Ali Jahanian
AU - Shokrgozar, Mohammad Ali
PY - 2009/11/1
Y1 - 2009/11/1
N2 - Environmental temperature variations are the most common stresses experienced by a wide range of organisms. Lipocalin 2 (Lcn2/NGAL) is expressed in various normal and pathologic conditions. However, its precise functions have not been fully determined. Here we report the induction of Lcn2 by thermal stresses in vivo, and its role following exposure to cold and heat stresses in vitro. Induction of Lcn2 in liver, heart and kidney was detected by RT-PCR, Western blot and immunohistochemistry following exposure of mice to heat and cold stresses. When CHO and HEK293T cells overexpressing NGAL were exposed to cold stress, cell proliferation was higher compared to controls. Down-regulatrion of NGAL by siRNA in A549 cells resulted in less proliferation when exposed to cold stress compared to control cells. The number of apoptotic cells and expression of pro-apoptotic proteins were lower in the NGAL overexpressing CHO and HEK293T cells, but were higher in the siRNA-transfected A549 cells compared to controls, indicating that NGAL protects cells against cold stress. Following exposure of the cells to heat stress, ectopic expression of NGAL protected cells while addition of exogenous recombinant NGAL to the cell culture medium exacerbated the toxicity of heat stress specially when there was low or no endogenous expression of NGAL. It had a dual effect on apoptosis following heat stress. NGAL also increased the expression of HO-1. Lcn2/NGAL may have the potential to improve cell proliferation and preservation particularly to prevent cold ischemia injury of transplanted organs or for treatment of some cancers by hyperthermia. Crown
AB - Environmental temperature variations are the most common stresses experienced by a wide range of organisms. Lipocalin 2 (Lcn2/NGAL) is expressed in various normal and pathologic conditions. However, its precise functions have not been fully determined. Here we report the induction of Lcn2 by thermal stresses in vivo, and its role following exposure to cold and heat stresses in vitro. Induction of Lcn2 in liver, heart and kidney was detected by RT-PCR, Western blot and immunohistochemistry following exposure of mice to heat and cold stresses. When CHO and HEK293T cells overexpressing NGAL were exposed to cold stress, cell proliferation was higher compared to controls. Down-regulatrion of NGAL by siRNA in A549 cells resulted in less proliferation when exposed to cold stress compared to control cells. The number of apoptotic cells and expression of pro-apoptotic proteins were lower in the NGAL overexpressing CHO and HEK293T cells, but were higher in the siRNA-transfected A549 cells compared to controls, indicating that NGAL protects cells against cold stress. Following exposure of the cells to heat stress, ectopic expression of NGAL protected cells while addition of exogenous recombinant NGAL to the cell culture medium exacerbated the toxicity of heat stress specially when there was low or no endogenous expression of NGAL. It had a dual effect on apoptosis following heat stress. NGAL also increased the expression of HO-1. Lcn2/NGAL may have the potential to improve cell proliferation and preservation particularly to prevent cold ischemia injury of transplanted organs or for treatment of some cancers by hyperthermia. Crown
KW - Apoptosis
KW - Cold and heat stresses
KW - Cytoprotective factor
KW - Lcn2/NGAL
UR - http://www.scopus.com/inward/record.url?scp=70349782807&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349782807&partnerID=8YFLogxK
U2 - 10.1016/j.yexcr.2009.08.019
DO - 10.1016/j.yexcr.2009.08.019
M3 - Article
C2 - 19732769
AN - SCOPUS:70349782807
VL - 315
SP - 3140
EP - 3151
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 18
ER -
