Lipid-tubular network formation for biochemical reaction

Shin Ichiro M. Nomura, Kazunari Akiyoshi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we describe liposome connecting tubular networks as a chemical reactor system. Two types of cell-sized liposomes are prepared by natural swelling method. The one was encapsulating in vitro gene-expression system (without DNA) and the other was including plasmid DNA coding green fluorescent protein (GFP). After mixing them gently, ganglioside GM3 was added for the connecting liposomes to network formation. The gene expression was checked by confocal scanning fluorescent microscopy. Fluorescence recovery after photobleaching (FRAP) assay supports the diffusive transfer could be driven the reaction. Proteoliposome network formation was also demonstrated from in vitro synthesized membrane protein cytochrome b5 on liposomes. These result should be used for constructing membrane protein array or artificial-cellular reaction cluster,

Original languageEnglish
Title of host publication2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
Pages322-326
Number of pages5
DOIs
Publication statusPublished - 2007 Dec 1
Externally publishedYes
Event2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS - Nagoya, Japan
Duration: 2007 Nov 112007 Nov 14

Publication series

Name2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS

Other

Other2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
CountryJapan
CityNagoya
Period07/11/1107/11/14

ASJC Scopus subject areas

  • Artificial Intelligence
  • Computer Networks and Communications
  • Human-Computer Interaction
  • Electrical and Electronic Engineering

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  • Cite this

    Nomura, S. I. M., & Akiyoshi, K. (2007). Lipid-tubular network formation for biochemical reaction. In 2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS (pp. 322-326). [4420874] (2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS). https://doi.org/10.1109/MHS.2007.4420874