The source of unsubstituted etheno-adducts formed by the reaction between 4-oxo-2-nonenal (4-ONE) with DNA, was identified using liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry. The reaction of DNA with 13-hydroperoxyoctadecadienoic acid /vitamin C resulted in the initial generation of bifunctional electrophiles followed by the formation of etheno adducts. The mass spectrometer was operated in the positive ion mode with a corona discharge current of 5 μA. The results provide an important link between a primary product of lipid peroxidation and a mutagenic DNA-lesion that was detected in human tissue.
|Number of pages||2|
|Publication status||Published - 2002 Dec 1|
|Event||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States|
Duration: 2002 Jun 2 → 2002 Jun 6
|Other||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|Period||02/6/2 → 02/6/6|
ASJC Scopus subject areas