Lipid hydroperoxide-derived modifications to cellular macromolecules: From DNA to proteomics

Ian A. Blair, Tomoyuki Oe, Seon Hwa Lee

Research output: Contribution to conferencePaperpeer-review

Abstract

The source of unsubstituted etheno-adducts formed by the reaction between 4-oxo-2-nonenal (4-ONE) with DNA, was identified using liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry. The reaction of DNA with 13-hydroperoxyoctadecadienoic acid /vitamin C resulted in the initial generation of bifunctional electrophiles followed by the formation of etheno adducts. The mass spectrometer was operated in the positive ion mode with a corona discharge current of 5 μA. The results provide an important link between a primary product of lipid peroxidation and a mutagenic DNA-lesion that was detected in human tissue.

Original languageEnglish
Pages71-72
Number of pages2
Publication statusPublished - 2002 Dec 1
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 22002 Jun 6

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL
Period02/6/202/6/6

ASJC Scopus subject areas

  • Spectroscopy

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