Lipid hydroperoxide-derived 2′-deoxycytidine adducts

Michael Pollack; Seon Hwa Lee; Tomoyuki Oe; Ian A. Blair

Lipid hydroperoxide-derived 2′-deoxycytidine adducts

Michael Pollack, Seon Hwa Lee, Tomoyuki Oe, Ian A. Blair

Research output: Contribution to conference › Paper › peer-review

Abstract

The covalent modification of 2′-deoxycytidine (dCyd) by genotoxic bifunctional electrophiles, to produce unsubstituted and substituted etheno-dCyd adducts was discussed. The presence of DNA-adducts increased the probability of errors during DNA replication. The decomposition of 13- hydroperoxyoctadecadienoic acid (13-HPODE) was analyzed by reverse-phase chromatography with an acetonitrile/water system. The results of analysis show that the decomposition of 13-HPODE in the presence of dCyd by Liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry revealed the formation of the unsubstituted 3,N ⁴-etheno-dCyd adduct and two substituted etheno-dCyd adducts.

Original language	English
Pages	633-634
Number of pages	2
Publication status	Published - 2002 Dec 1
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2002 Jun 2 → 2002 Jun 6

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country	United States
City	Orlando, FL
Period	02/6/2 → 02/6/6

ASJC Scopus subject areas

Spectroscopy

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title = "Lipid hydroperoxide-derived 2′-deoxycytidine adducts",

abstract = "The covalent modification of 2′-deoxycytidine (dCyd) by genotoxic bifunctional electrophiles, to produce unsubstituted and substituted etheno-dCyd adducts was discussed. The presence of DNA-adducts increased the probability of errors during DNA replication. The decomposition of 13- hydroperoxyoctadecadienoic acid (13-HPODE) was analyzed by reverse-phase chromatography with an acetonitrile/water system. The results of analysis show that the decomposition of 13-HPODE in the presence of dCyd by Liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry revealed the formation of the unsubstituted 3,N 4-etheno-dCyd adduct and two substituted etheno-dCyd adducts.",

author = "Michael Pollack and Lee, {Seon Hwa} and Tomoyuki Oe and Blair, {Ian A.}",

year = "2002",

month = dec,

day = "1",

language = "English",

pages = "633--634",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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T1 - Lipid hydroperoxide-derived 2′-deoxycytidine adducts

AU - Pollack, Michael

AU - Lee, Seon Hwa

AU - Oe, Tomoyuki

AU - Blair, Ian A.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The covalent modification of 2′-deoxycytidine (dCyd) by genotoxic bifunctional electrophiles, to produce unsubstituted and substituted etheno-dCyd adducts was discussed. The presence of DNA-adducts increased the probability of errors during DNA replication. The decomposition of 13- hydroperoxyoctadecadienoic acid (13-HPODE) was analyzed by reverse-phase chromatography with an acetonitrile/water system. The results of analysis show that the decomposition of 13-HPODE in the presence of dCyd by Liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry revealed the formation of the unsubstituted 3,N 4-etheno-dCyd adduct and two substituted etheno-dCyd adducts.

AB - The covalent modification of 2′-deoxycytidine (dCyd) by genotoxic bifunctional electrophiles, to produce unsubstituted and substituted etheno-dCyd adducts was discussed. The presence of DNA-adducts increased the probability of errors during DNA replication. The decomposition of 13- hydroperoxyoctadecadienoic acid (13-HPODE) was analyzed by reverse-phase chromatography with an acetonitrile/water system. The results of analysis show that the decomposition of 13-HPODE in the presence of dCyd by Liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry revealed the formation of the unsubstituted 3,N 4-etheno-dCyd adduct and two substituted etheno-dCyd adducts.

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UR - http://www.scopus.com/inward/citedby.url?scp=2442672000&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442672000

SP - 633

EP - 634

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Lipid hydroperoxide-derived 2′-deoxycytidine adducts

Abstract

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ASJC Scopus subject areas

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