Lipid bilayer array for simultaneous recording of ion channel activities

Ayumi Hirano-Iwata, Tomohiro Nasu, Azusa Oshima, Yasuo Kimura, Michio Niwano

Research output: Contribution to journalReview articlepeer-review

9 Citations (Scopus)

Abstract

This paper describes an array of stable and reduced-solvent bilayer lipid membranes (BLMs) formed in microfabricated silicon chips. BLMs were first vertically formed simultaneously and then turned 90° in order to realize a horizontal BLM array. Since the present BLMs are mechanically stable and robust, the BLMs survive this relatively tough process. Typically, a ∼60 yield in simultaneous BLM formation over 9 sites was obtained. Parallel recordings of gramicidin channel activities from different BLMs were demonstrated. The present system has great potential as a platform of BLM-based high throughput drug screening for ion channel proteins.

Original languageEnglish
Article number023702
JournalApplied Physics Letters
Volume101
Issue number2
DOIs
Publication statusPublished - 2012 Jul 9

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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