Lipid accumulation and foam cell formation in chinese hamster ovary cells overexpressing very low density lipoprotein receptor

Jinya Suzuki, Sadao Takahashi, Koji Oida, Akihiko Shimada, Mitsuyuki Kohno, Toshitaka Tamai, Susumu Miyabo, Tokuo Yamamoto, Tsuguhiko Nakai

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

The rabbit very low density lipoprotein receptor gene was introduced into LDL receptor-negative Chinese hamster ovary cells (ldl-A7). Incubation of the transfected cells with rabbit β-VLDL (5 to 8 μg protein/ml), for 6 days, induced foam cell formation. The cells accumulated lipid droplets visualized by oil red-O staining; the cellular cholesteryl ester and triglyceride content increased two- to threefold. [3H]Oleate incorporation into cholesteryl [3H]oleate was stimulated threefold by incubation of cells with β-VLDL (20 μg protein/ml). Northern blot analysis revealed the presence of VLDL receptor mRNA in rabbit resident alveolar macrophages. Incubation of the macrophages with β-VLDL (20 μg protein/ml) for 24 hours induced foam cell formation but had virtually no effect on VLDL receptor mRNA abundance. These results suggest that the VLDL receptor on macrophages may play an important role in foam cell formation.

Original languageEnglish
Pages (from-to)835-842
Number of pages8
JournalBiochemical and biophysical research communications
Volume206
Issue number3
DOIs
Publication statusPublished - 1995 Jan 26

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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