TY - JOUR
T1 - Lipid accumulation and foam cell formation in chinese hamster ovary cells overexpressing very low density lipoprotein receptor
AU - Suzuki, Jinya
AU - Takahashi, Sadao
AU - Oida, Koji
AU - Shimada, Akihiko
AU - Kohno, Mitsuyuki
AU - Tamai, Toshitaka
AU - Miyabo, Susumu
AU - Yamamoto, Tokuo
AU - Nakai, Tsuguhiko
PY - 1995/1/26
Y1 - 1995/1/26
N2 - The rabbit very low density lipoprotein receptor gene was introduced into LDL receptor-negative Chinese hamster ovary cells (ldl-A7). Incubation of the transfected cells with rabbit β-VLDL (5 to 8 μg protein/ml), for 6 days, induced foam cell formation. The cells accumulated lipid droplets visualized by oil red-O staining; the cellular cholesteryl ester and triglyceride content increased two- to threefold. [3H]Oleate incorporation into cholesteryl [3H]oleate was stimulated threefold by incubation of cells with β-VLDL (20 μg protein/ml). Northern blot analysis revealed the presence of VLDL receptor mRNA in rabbit resident alveolar macrophages. Incubation of the macrophages with β-VLDL (20 μg protein/ml) for 24 hours induced foam cell formation but had virtually no effect on VLDL receptor mRNA abundance. These results suggest that the VLDL receptor on macrophages may play an important role in foam cell formation.
AB - The rabbit very low density lipoprotein receptor gene was introduced into LDL receptor-negative Chinese hamster ovary cells (ldl-A7). Incubation of the transfected cells with rabbit β-VLDL (5 to 8 μg protein/ml), for 6 days, induced foam cell formation. The cells accumulated lipid droplets visualized by oil red-O staining; the cellular cholesteryl ester and triglyceride content increased two- to threefold. [3H]Oleate incorporation into cholesteryl [3H]oleate was stimulated threefold by incubation of cells with β-VLDL (20 μg protein/ml). Northern blot analysis revealed the presence of VLDL receptor mRNA in rabbit resident alveolar macrophages. Incubation of the macrophages with β-VLDL (20 μg protein/ml) for 24 hours induced foam cell formation but had virtually no effect on VLDL receptor mRNA abundance. These results suggest that the VLDL receptor on macrophages may play an important role in foam cell formation.
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U2 - 10.1006/bbrc.1995.1119
DO - 10.1006/bbrc.1995.1119
M3 - Article
C2 - 7832794
AN - SCOPUS:0028878973
VL - 206
SP - 835
EP - 842
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -