Linear dominance relationship among four class-II S haplotypes in pollen is determined by the expression of SP11 in Brassica self-incompatibility

Tomohiro Kakizaki, Yoshinobu Takada, Akiko Ito, Go Suzuki, Hiroshi Shiba, Seiji Takayama, Akira Isogai, Masao Watanabe

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Self-incompatibility (SI) prevents self-fertilization by rejecting pollen from plants with the same S phenotype. The Brassica SI system is controlled sporophytically by multiple alleles at the single locus, S, and dominance relationships among S haplotypes are observed in both stigma and pollen. We have identified previously five different class-II S haplotypes in Brassica campestris. Here, we performed test-crosses between S heterozygotes and their respective parental S homozygotes for four of these class-II S haplotypes, and observed a linear dominance relationship on the pollen side. To determine how this relationship is controlled, we performed RNA gel blot analyses for six S heterozygotes and their respective parental S homozygotes using the corresponding SP11 clone as a probe. In all six S heterozygotes, SP11 derived from a dominant haplotype was predominantly expressed, and SP11 derived from a recessive haplotype was repressed. Thus, the linear dominance relationship of the SI phenotype on the pollen side is regulated by the expression of SP11.

Original languageEnglish
Pages (from-to)70-75
Number of pages6
JournalPlant and Cell Physiology
Volume44
Issue number1
DOIs
Publication statusPublished - 2003 Jan 1
Externally publishedYes

Keywords

  • Brassica campestris (syn. rapa)
  • Linear dominance relationship
  • Monoallelic expression
  • S multiple alleles
  • SP11
  • Self-incompatibility

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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