Limulus test (factor G) and polysaccharides from fungus

T. Miyazaki, S. Kohno, K. Mitsutake, S. Maesaki, H. Yamada, A. Yasuoka, K. Sasayama, Mitsuo Kaku, H. Koga, K. Hara

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Abstract

Picogram quantifies per ml of endotoxin showed a positive limulus test. The concentration more than ten pg/ml of curdlan ((1-3)-beta-D-glucan) was also positive to a conventional limulus test, which contains a factor G. One nanogram per ml of CM-curdlan was positive, but higher concentration of 10 pg/ml of CM-curdlan decreased its optical density of the conventional limulus test. More than one hundred ng/ml of mannan from Saccharomyces cerevisiae did not react with factor G. However, a culture supernatant of Saccharomyces cerevisiae activated factor G. This result suggested that beta-glucan activated factor G. In addition, culture supernatant of Aspergillus fumigatus, Rhodotorula rubra, Candida parapsilosis, Candida tropicalis, Candida krusei also activated factor G, but Cryptococcus neoformans did not activate it.

Original languageEnglish
Pages (from-to)1030-1036
Number of pages7
JournalKansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
Volume66
Issue number8
DOIs
Publication statusPublished - 1992 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Miyazaki, T., Kohno, S., Mitsutake, K., Maesaki, S., Yamada, H., Yasuoka, A., Sasayama, K., Kaku, M., Koga, H., & Hara, K. (1992). Limulus test (factor G) and polysaccharides from fungus. Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, 66(8), 1030-1036. https://doi.org/10.11150/kansenshogakuzasshi1970.66.1030