Limited intercellular spread of spontaneous Ca2+ signals via gap junctions between mouse chromaffin cells in situ

Kazuhiko Yamagami, Takashi Moritoyo, Minoru Wakamori, Masaru Sorimachi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Using a confocal laser-scanning microscope, we measured the changes in the cytosolic Ca2+ concentration ([Ca]i) of chromaffin cells on adrenal slice preparations of mouse. The spontaneous fluctuations of [Ca]i were often observed in situ, as reported in isolated rat and bovine cells. Intriguingly, the spontaneous [Ca]i changes in one cell were often transmitted to one or two adjacent cells, and the synchronized [Ca]i changes were often observed in two adjacent cells, both of which failed to respond to ATP. The synchronized [Ca]i changes between two cells were much less frequently observed in rat than in mouse adrenals. The spontaneous [Ca]i changes were also synchronized between closely touching mouse chromaffin cells in culture. These results suggest that the spread of Ca2+ signaling is mediated by low-conductance intercellular coupling.

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalNeuroscience Letters
Volume323
Issue number2
DOIs
Publication statusPublished - 2002 Apr 26
Externally publishedYes

Keywords

  • ATP
  • Confocal laser-scanning microscope
  • Fura-2 microfluorometry
  • Gap junctions
  • Mouse adrenal chromaffin cells
  • Oregon green BAPTA-1/AM
  • The cytosolic Ca concentration

ASJC Scopus subject areas

  • Neuroscience(all)

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