Limitations to photosynthesis by proton motive force-induced photosystem II photodamage

Geoffry A. Davis, Atsuko Kanazawa, Mark Aurel Schöttler, Kaori Kohzuma, John E. Froehlich, A. William Rutherford, Mio Satoh-Cruz, Deepika Minhas, Stefanie Tietz, Amit Dhingra, David M. Kramer

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

The thylakoid proton motive force (pmf) generated during photosynthesis is the essential driving force for ATP production; it is also a central regulator of light capture and electron transfer. We investigated the effects of elevated pmf on photosynthesis in a library of Arabidopsis thaliana mutants with altered rates of thylakoid lumen proton efflux, leading to a range of steady- state pmf extents. We observed the expected pmf-dependent alterations in photosynthetic regulation, but also strong effects on the rate of photosystem II (PSII) photodamage. Detailed analyses indicate this effect is related to an elevated electric field (∆ψ) component of the pmf, rather than lumen acidification, which in vivo increased PSII charge recombination rates, producing singlet oxygen and subsequent photodamage. The effects are seen even in wild type plants, especially under fluctuating illumination, suggesting that ∆ψ -induced photodamage represents a previously unrecognized limiting factor for plant productivity under dynamic environmental conditions seen in the field.

Original languageEnglish
Article numbere16921
Pages (from-to)23-27
Number of pages5
JournaleLife
Volume5
Issue numberOCTOBER2016
DOIs
Publication statusPublished - 2016 Oct

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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