Light-addressable potentiometric sensor (LAPS) combined with magnetic beads for pharmaceutical screening

Torsten Wagner, Wolfgang Vornholt, Carl Frederik Benedikt Werner, Tatsuo Yoshinobu, Ko ichiro Miyamoto, Michael Keusgen, Michael J. Schöning

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The light-addressable potentiometric sensor (LAPS) has the unique feature to address different regions of a sensor surface without the need of complex structures. Measurements at different locations on the sensor surface can be performed in a common analyte solution, which distinctly simplifies the fluidic set-up. However, the measurement in a single analyte chamber prevents the application of different drugs or different concentrations of a drug to each measurement spot at the same time as in the case of multi-reservoir-based set-ups. In this work, the authors designed a LAPS-based set-up for cell culture screening that utilises magnetic beads loaded with the endotoxin (lipopolysaccharides, LPS), to generate a spatially distributed gradient of analyte concentration. Different external magnetic fields can be adjusted to move the magnetic beads loaded with a specific drug within the measurement cell. By recording the metabolic activities of a cell layer cultured on top of the LAPS surface, this work shows the possibility to apply different concentrations of a sample along the LAPS measurement spots within a common analyte solution.

Original languageEnglish
Pages (from-to)2-7
Number of pages6
JournalPhysics in Medicine
Volume1
DOIs
Publication statusPublished - 2016 Jun 1

Keywords

  • Biosensor
  • Field-effect
  • LAPS
  • Lipopolysaccharides (LPS)
  • Macrophages
  • Magnetic beads

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Instrumentation

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