Light activation of expression associated with the tomato rbcS promoter in transformed tobacco cell line BY-2

Nobuyuki Uozumi, Yoshihisa Inoue, Ken ichi Yamazaki, Takeshi Kobayashi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The promoter (2.3 kb) of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcS3B) from tomato was inserted into the upstream multi-cloning site of β-glucuronidase (GUS) gene. The construction was introduced into the tobacco suspension cultured cell, BY-2. The transformed tobacco cell exhibited greater GUS activity (10-fold) under the light condition than the dark condition. The specific GUS activity increased with increasing culture time under the light condition. In fed-batch culture, high density culture was achieved, 47.6 g l-1 dry weight, which was 6.8-fold greater than the batch culture, whereas the GUS was produced 1.7-fold in specific activity compared with the batch culture. Growth of BY-2 and GUS gene expression during the culture were simulated by a kinetic model.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalJournal of Biotechnology
Volume36
Issue number1
DOIs
Publication statusPublished - 1994 Jul 29

Keywords

  • BY-2
  • GUS activity
  • Light inducible promoter
  • Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit
  • Tobacco cell line

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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