Lgr4-deficient mice showed premature differentiation of ureteric bud with reduced expression of Wnt effector Lef1 and Gata3

Yasuaki Mohri, Kazunori Oyama, Atsushi Akamatsu, Shigeki Kato, Katsuhiko Nishimori

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We previously reported that Lgr4 has a critical role in the morphogenesis of kidney, but the detailed functions of Lgr4 in kidney development have not been elucidated. In contrast to Lgr4 null mice with 129Ola × C57BL/6J mixed background, C57BL/6J-backcrossed Lgr4 null mice (Lgr4-/-) showed the severe phenotype of embryonic lethality and also had dilated tubules in kidneys at E16.5. Based on quantitative RT-PCR and in situ hybridization, branching morphogenesis at E15.5 in the Lgr4-/- was arrested earlier, and both DBA-lectin staining and immunohistochemical analysis using Aqp3 antibodies showed that the ureteric bud (UB) of Lgr4-/- kidneys underwent premature differentiation. Furthermore, quantitative RT-PCR and histological analysis suggested that the impaired UB differentiation was caused by down-regulation of the Wnt pathway and Gata3 in the Lgr4-/- kidneys. We demonstrate here that Lgr4 has a novel function for maintaining the UB in an undifferentiated state.

Original languageEnglish
Pages (from-to)1626-1634
Number of pages9
JournalDevelopmental Dynamics
Volume240
Issue number6
DOIs
Publication statusPublished - 2011 Jun 1

Keywords

  • Kidney morphogenesis
  • Knockout mice
  • Lgr4
  • Ureteric bud
  • Wnt canonical pathway

ASJC Scopus subject areas

  • Developmental Biology

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