Leydig cell tumor of the testis: Analysis of testosterone production and secretion by three-dimensional histoculture

Hironobu Sasano, Ikuo Maehara, Junji Ueno, Seiichi Orikasa, Hiroshi Nagura

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We treated an 11-year-old boy with a testicular Leydig cell tumor. We analyzed the testosterone production of this tumor by immunolocalization of steroidogenic enzymes and in vitro three-dimensional histoculture. Spermatic venous blood from the tumor bearing testis had noticeably high concentrations of testosterone and androstenedione. The tumor had the characteristic ultrastructural features of steroid producing cells and was immunoreactive for P450scc (side chain cleavage), 3βHSD (hydroxysteroid dehydrogenase) and P450c17 (17α-hydroxylase). Three-dimensional collagen-gel-supported histoculture demonstrated that the tumor tissue in the culture maintained its histologic architecture, expression of steroidogenic enzymes, and secretion of testosterone into the medium for up to 7 days in culture. Histoculture preserved in vitro testosterone production in this case of testicular Leydig cell tumor.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
Journalendocrine journal
Volume43
Issue number1
DOIs
Publication statusPublished - 1996 Feb

Keywords

  • Histoculture
  • Immunohistochemistry
  • Leydig cell tumor
  • Testis
  • Testosterone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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