Levels of Periplasmic Nitrate Reductase during Denitrification are Lower in Bradyrhizobium japonicum than in Bradyrhizobium diazoefficiens

Arthur Fernandes Siqueira, Masayuki Sugawara, Haruka Arashida, Kiwamu Minamisawa, Cristina Sánchez

Research output: Contribution to journalArticle

Abstract

Soybean plants host endosymbiotic dinitrogen (N2)-fixing bacteria from the genus Bradyrhizobium. Under oxygen-limiting conditions, Bradyrhizobium diazoefficiens and Bradyrhizobium japonicum perform denitrification by sequentially reducing nitrate (NO3-) to nitrous oxide (N2O) or N2. The anaerobic reduction of NO3- to N2O was previously shown to be lower in B. japonicum than in B. diazoefficiens due to impaired periplasmic nitrate reductase (Nap) activity in B. japonicum. We herein demonstrated that impaired Nap activity in B. japonicum was due to low Nap protein levels, which may be related to a decline in the production of FixP and FixO proteins by the cbb3-type oxidase.

Original languageEnglish
JournalMicrobes and environments
Volume35
Issue number3
DOIs
Publication statusPublished - 2020

Keywords

  • Bradyrhizobium
  • cbb3 oxidase
  • competitive growth
  • denitrification
  • periplasmic nitrate reductase

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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