TY - JOUR
T1 - Levels of Anti-Retinal Antibodies in Retinal Detachment and Proliferative Vitreoretinopathy
AU - Ichinohasama, Reo
AU - Nishiguchi, Koji M.
AU - Fujita, Kosuke
AU - Aizawa, Naoko
AU - Inoue, Takashi
AU - Sasaki, Erika
AU - Kunikata, Hiroshi
AU - Nakazawa, Toru
PY - 2018/6/3
Y1 - 2018/6/3
N2 - Purpose: The purpose of the study is to investigate the correlation between intraocular anti-retinal antibodies and clinical measurements in patients with rhegmatogenous retinal detachment (RRD) and proliferative vitreoretinopathy (PVR). Material and methods: Aqueous humor and vitreous samples were collected from patients with RRD, PVR, and from control subjects with macular hole. The levels of total protein (TP), IgG, and anti-retinal antibodies were determined with a bicinchoninic acid assay, enzyme-linked immunosorbent assay, and dot blot, respectively. Correlations between these measurements were assessed using Pearson’s correlation test. Analysis of variance followed by a post-hoc test or the Student t-test was used to compare differences between groups. Results: The levels of anti-retinal antibodies and IgG were correlated with each other (P < 0.010). The IgG concentration was higher in patients with PVR than in controls in both the aqueous humor (P < 0.001) and the vitreous (P < 0.001), but not in patients with RRD. Conversely, TP levels and anti-retinal antibodies in both ocular fluids from RRD and PVR patients did not significantly differ from the controls. In a subgroup analysis, vitreal anti-retinal antibody levels were correlated with average macular thickness in the re-attached macula following surgery for macula-off RRD/PVR (P = 0.012). Furthermore, patients with post-operative cystoid macular edema had a higher level of vitreal anti-retinal antibodies than those without (P = 0.009). Conclusions: Intravitreal anti-retinal antibodies were increased in the eyes with maculopathy after surgical intervention for RRD/PVR.
AB - Purpose: The purpose of the study is to investigate the correlation between intraocular anti-retinal antibodies and clinical measurements in patients with rhegmatogenous retinal detachment (RRD) and proliferative vitreoretinopathy (PVR). Material and methods: Aqueous humor and vitreous samples were collected from patients with RRD, PVR, and from control subjects with macular hole. The levels of total protein (TP), IgG, and anti-retinal antibodies were determined with a bicinchoninic acid assay, enzyme-linked immunosorbent assay, and dot blot, respectively. Correlations between these measurements were assessed using Pearson’s correlation test. Analysis of variance followed by a post-hoc test or the Student t-test was used to compare differences between groups. Results: The levels of anti-retinal antibodies and IgG were correlated with each other (P < 0.010). The IgG concentration was higher in patients with PVR than in controls in both the aqueous humor (P < 0.001) and the vitreous (P < 0.001), but not in patients with RRD. Conversely, TP levels and anti-retinal antibodies in both ocular fluids from RRD and PVR patients did not significantly differ from the controls. In a subgroup analysis, vitreal anti-retinal antibody levels were correlated with average macular thickness in the re-attached macula following surgery for macula-off RRD/PVR (P = 0.012). Furthermore, patients with post-operative cystoid macular edema had a higher level of vitreal anti-retinal antibodies than those without (P = 0.009). Conclusions: Intravitreal anti-retinal antibodies were increased in the eyes with maculopathy after surgical intervention for RRD/PVR.
KW - Proliferative vitreoretinopathy
KW - aqueous humor
KW - autoantibodies
KW - retinal detachment
KW - vitreous
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U2 - 10.1080/02713683.2018.1451544
DO - 10.1080/02713683.2018.1451544
M3 - Article
C2 - 29547015
AN - SCOPUS:85044041657
VL - 43
SP - 804
EP - 809
JO - Current Eye Research
JF - Current Eye Research
SN - 0271-3683
IS - 6
ER -