Leukotriene B4 ω-hydroxylase activity in polymorphonuclear leukocytes from patients with inflammatory bowel disease

A. Ikehata, N. Hiwatashi, Yoshitaka Kinouchi, K. Ito, H. Yamazaki, T. Toyota

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

ω-oxidation is regarded as the major pathway for the catabolism of leukotriene B4 (LTB4). To determine how LTB4 ω-hydroxylase is modulated in inflammatory bowel disease, we investigated the kinetic characteristics of this enzyme in 10 patients with Crohn's disease (CD), nine with ulcerative colitis (UC) and eight healthy control subjects. After incubating polymorphonuclear leukocytes with various concentrations of 3H-labeled LTB4, ω-oxidation products were serapated by high performance liquid chromatography (HPLC) and the radioactivity was measured by a liquid scintilation counter. The apparent Vmax values were significantly higher in both disease than in healthy control subjects, although the difference between CD and UC was insignificant. There was no difference in the apparent Km values. And the Vmax Km ratios of patients with CD were significantly higher than that of healthy control subjects. It is suggested that LTB4 metabolism is activated in inflammatory bowel disease (IBD) and that the modulation of this enzyme activity has an important role in the pathogenesis of inflammatory bowel disease.

Original languageEnglish
Pages (from-to)489-494
Number of pages6
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume49
Issue number1
DOIs
Publication statusPublished - 1993 Jan 1

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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