Leukotoxin family genes in Staphylococcus aureus isolated from domestic animals and prevalence of lukM-lukF-PV genes by bacteriophages in bovine isolates

Tomoko Yamada, Naoko Tochimaru, Sachiko Nakasuji, Eiji Hata, Hideki Kobayashi, Masashi Eguchi, Jun Kaneko, Yoshiyuki Kamio, Toshio Kaidoh, Shotaro Takeuchi

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Leukotoxin family genes in Staphylococcus aureus isolated from domestic animals were examined by polymerase chain reaction. LukS and lukF genes were detected in all 48 avian and 72 porcine isolates of S. aureus. LukE and lukD genes, located in a putative staphylococcal pathogenicity island (Sapln3/Saplm3), were recognized in 44 (91.7%) of 48 avian isolates, but these genes were not detected in porcine isolates. In 297 bovine isolates collected from mastitic cow's milk and bulk milk from dairy farms in two regions, lukM and lukF-PV(P83) genes in addition to lukS-lukF and lukE-lukD genes were detected in 100 (62.5%) of the 160 isolates from Ishikawa and in118 (86.1%) of the 137 isolates from Hokkaido. When the lysogeny of S. aureus bovine isolates was examined by treatment with mitomycin C, clearing of the culture due to cell lysis was observed in 34 (91.9%) of 37 lukM-lukF-PV(P83) genes - positive isolates. In addition, we isolated a novel lukM-lukF-PV(P83)-carrying (designated φLukM), and revealed that the lukM-lukF-PV(P83) genes were located very close to an amidase gene on the temperate phage genomes. These results suggest horizontal transmission of lukM-lukF-PV(P83) genes by temperate bacteriophages in S. aureus of bovine origin.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalVeterinary Microbiology
Volume110
Issue number1-2
DOIs
Publication statusPublished - 2005 Sep 30

Keywords

  • Bovine mastitis
  • Leukotoxin genes
  • Staphylococcus aureus
  • Temperate phage

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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