TY - JOUR
T1 - Leptospiral lipopolysaccharide stimulates the expression of toll-like receptor 2 and cytokines in pig fibroblasts
AU - Guo, Yijie
AU - Fukuda, Tomokazu
AU - Donai, Kenichiro
AU - Kuroda, Kengo
AU - Masuda, Mizuki
AU - Nakamura, Shuichi
AU - Yoneyama, Hiroshi
AU - Isogai, Emiko
N1 - Publisher Copyright:
© 2014 Japanese Society of Animal Science.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Pigs throughout the world are afflicted with leptospirosis, causing serious economic losses and potential hazards to human health. Although it has been known that leptospiral lipopolysaccharide (L-LPS) is involved in an immunological reaction between an antigen and a host cell, little is known about how the immune system of pigs can respond to L-LPS. Here, we stimulated pig fibroblasts by L-LPS and then quantitatively measured gene and protein expression levels of two toll-like receptors (TLRs), TLR2 and TLR4, by real-time PCR and Western blotting. As a result, expression of TLR2 was found to be significantly up-regulated within 24h after L-LPS stimulation whereas induction of TLR4 expression was relatively weak. We also revealed that of myeloid differentiation primary response gene 88 (MyD88), interleukin 6 (IL-6) and IL-8 gene expressions were markedly up-regulated by L-LPS stimulation. These results may suggest that the pig cell can activate TLR2 rather than TLR4 by L-LPS stimulation, thereby inducing expression of cytokines.
AB - Pigs throughout the world are afflicted with leptospirosis, causing serious economic losses and potential hazards to human health. Although it has been known that leptospiral lipopolysaccharide (L-LPS) is involved in an immunological reaction between an antigen and a host cell, little is known about how the immune system of pigs can respond to L-LPS. Here, we stimulated pig fibroblasts by L-LPS and then quantitatively measured gene and protein expression levels of two toll-like receptors (TLRs), TLR2 and TLR4, by real-time PCR and Western blotting. As a result, expression of TLR2 was found to be significantly up-regulated within 24h after L-LPS stimulation whereas induction of TLR4 expression was relatively weak. We also revealed that of myeloid differentiation primary response gene 88 (MyD88), interleukin 6 (IL-6) and IL-8 gene expressions were markedly up-regulated by L-LPS stimulation. These results may suggest that the pig cell can activate TLR2 rather than TLR4 by L-LPS stimulation, thereby inducing expression of cytokines.
KW - Leptospiral lipopolysaccharide
KW - Pig fibroblasts
KW - Toll-like receptor 2
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U2 - 10.1111/asj.12254
DO - 10.1111/asj.12254
M3 - Article
C2 - 25039909
AN - SCOPUS:84921880490
VL - 86
SP - 238
EP - 244
JO - Animal Science Journal
JF - Animal Science Journal
SN - 1344-3941
IS - 2
ER -