Leptospiral lipopolysaccharide stimulates the expression of toll-like receptor 2 and cytokines in pig fibroblasts

Yijie Guo, Tomokazu Fukuda, Kenichiro Donai, Kengo Kuroda, Mizuki Masuda, Shuichi Nakamura, Hiroshi Yoneyama, Emiko Isogai

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Pigs throughout the world are afflicted with leptospirosis, causing serious economic losses and potential hazards to human health. Although it has been known that leptospiral lipopolysaccharide (L-LPS) is involved in an immunological reaction between an antigen and a host cell, little is known about how the immune system of pigs can respond to L-LPS. Here, we stimulated pig fibroblasts by L-LPS and then quantitatively measured gene and protein expression levels of two toll-like receptors (TLRs), TLR2 and TLR4, by real-time PCR and Western blotting. As a result, expression of TLR2 was found to be significantly up-regulated within 24h after L-LPS stimulation whereas induction of TLR4 expression was relatively weak. We also revealed that of myeloid differentiation primary response gene 88 (MyD88), interleukin 6 (IL-6) and IL-8 gene expressions were markedly up-regulated by L-LPS stimulation. These results may suggest that the pig cell can activate TLR2 rather than TLR4 by L-LPS stimulation, thereby inducing expression of cytokines.

Original languageEnglish
Pages (from-to)238-244
Number of pages7
JournalAnimal Science Journal
Issue number2
Publication statusPublished - 2015 Feb 1


  • Leptospiral lipopolysaccharide
  • Pig fibroblasts
  • Toll-like receptor 2

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)


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