Lectin cytochemistry of the lacrimal sac epithelium in experimental dacryocystitis

Seiji Maeda, Makoto Ishikawa, Tohru Abe, Seiki Konno, Shozo Sakuragi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Purpose: To study the glycoconjugates in the lacrimal sac epithelium of Japanese white rabbits with experimentally induced chronic dacryocystitis. Methods: Chronic dacryocystitis was induced by a subcutaneous injection of albumin followed by an injection of Staphylococcus aureus into the lacrimal sac. The histological appearance of the lacrimal sac was studied using the alcian blue-periodic acid-Schiff sequence. In addition, the specific binding to the lacrimal sac epithelium of Ulex europaeus agglutinin 1, Ricinus communis agglutinin 1, peanut agglutinin, and soybean agglutinin was also studied. Results: Staining with alcian blue-periodic acid-Schiff sequence showed hyperplasia of the goblet cells in the inflamed lacrimal sac epithelium. Lectin cytochemistry revealed specific binding of Ulex europaeus agglutinin 1, Ricinus communis agglutinin 1, peanut agglutinin, and soybean agglutinin to the lacrimal sac epithelium. Conclusions: These results indicated that the composition of glycoconjugates in the lacrimal sac epithelium is markedly changed in dacryocystitis. There seems to be a fundamental abnormality in glycoconjugate synthesis in the chronically inflamed lacrimal sac epithelium.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalJapanese Journal of Ophthalmology
Volume43
Issue number2
DOIs
Publication statusPublished - 1999 Mar
Externally publishedYes

Keywords

  • Experimental dacryocystitis
  • Glycoconjugates
  • Lectin cytochemistry
  • Rabbit

ASJC Scopus subject areas

  • Ophthalmology

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