Leaf discs floated on water are different from intact leaves in photosynthesis and photoinhibition

Masaharu C. Kato, Kouki Hikosaka, Tadaki Hirose

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Photoinhibition has been often evaluated with leaf discs floated on water or placed on wet papers to prevent desiccation. Under these conditions, there is a possibility that CO2 diffusion is blocked by water, which may lead to reduction in photosynthetic CO2 assimilation. Using Chenopodium album L. grown at two irradiances, photosynthesis, quantum yield of Photosystem II (ΔF/Fm′), non-photochemical quenching (qN), and photoinhibition were compared between detached leaves and leaf discs. In low-light-grown plants, photoinhibition was greater in leaf discs than in detached leaves, while in high-light-grown plants, there was little difference. Leaf discs showed lower rates of photosynthesis and ΔF/Fm′, and higher qN. The ΔF/Fm′ in leaf discs increased when leaf discs were exposed to high concentration of CO2, suggesting that CO2 diffusion to chloroplasts was limited in leaf discs floated on water.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalPhotosynthesis Research
Volume72
Issue number1
DOIs
Publication statusPublished - 2002 Aug 7

Keywords

  • Chenopodium album L.
  • Chlorophyll fluorescence
  • Detached leaf
  • Leaf disc
  • Photoinhibition

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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