TY - JOUR
T1 - Leaf discs floated on water are different from intact leaves in photosynthesis and photoinhibition
AU - Kato, Masaharu C.
AU - Hikosaka, Kouki
AU - Hirose, Tadaki
N1 - Funding Information:
We thank K. Sato, T. Yamano, and R. Oguchi for help in growing plants, S. Saori for stomatal data, and anonymous reviewers for valuable suggestions. This study was supported in part by grants-in-aid from the Minister of Education, Science and Culture of Japan.
PY - 2002
Y1 - 2002
N2 - Photoinhibition has been often evaluated with leaf discs floated on water or placed on wet papers to prevent desiccation. Under these conditions, there is a possibility that CO2 diffusion is blocked by water, which may lead to reduction in photosynthetic CO2 assimilation. Using Chenopodium album L. grown at two irradiances, photosynthesis, quantum yield of Photosystem II (ΔF/Fm′), non-photochemical quenching (qN), and photoinhibition were compared between detached leaves and leaf discs. In low-light-grown plants, photoinhibition was greater in leaf discs than in detached leaves, while in high-light-grown plants, there was little difference. Leaf discs showed lower rates of photosynthesis and ΔF/Fm′, and higher qN. The ΔF/Fm′ in leaf discs increased when leaf discs were exposed to high concentration of CO2, suggesting that CO2 diffusion to chloroplasts was limited in leaf discs floated on water.
AB - Photoinhibition has been often evaluated with leaf discs floated on water or placed on wet papers to prevent desiccation. Under these conditions, there is a possibility that CO2 diffusion is blocked by water, which may lead to reduction in photosynthetic CO2 assimilation. Using Chenopodium album L. grown at two irradiances, photosynthesis, quantum yield of Photosystem II (ΔF/Fm′), non-photochemical quenching (qN), and photoinhibition were compared between detached leaves and leaf discs. In low-light-grown plants, photoinhibition was greater in leaf discs than in detached leaves, while in high-light-grown plants, there was little difference. Leaf discs showed lower rates of photosynthesis and ΔF/Fm′, and higher qN. The ΔF/Fm′ in leaf discs increased when leaf discs were exposed to high concentration of CO2, suggesting that CO2 diffusion to chloroplasts was limited in leaf discs floated on water.
KW - Chenopodium album L.
KW - Chlorophyll fluorescence
KW - Detached leaf
KW - Leaf disc
KW - Photoinhibition
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U2 - 10.1023/A:1016097312036
DO - 10.1023/A:1016097312036
M3 - Article
AN - SCOPUS:0036329030
VL - 72
SP - 65
EP - 70
JO - Photosynthesis Research
JF - Photosynthesis Research
SN - 0166-8595
IS - 1
ER -