LCD-CCD synchronization detection for fluorescence polarization immunoassay

Osamu Wakao, Yusaku Fujii, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A new fluorescence polarization (FP) measurement system applicable to FP immunoassay (FPIA) was developed. The detection principle of the system is unique and is based on the synchronization detection between the orientation of the liquid-crystal (LC) molecules of a LC display (LCD) and the sampling frequency of a CCD camera. In this paper, we demonstrate the viscosity dependence of FP of fluorescein for a proof-of-concept and FPIA of an antibiotic (chloramphenicol). These results showed that our system had a great potential for simultaneous multi-sample detection. Furthermore, our system can perform miniaturization and cost reduction easily.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages2271-2273
Number of pages3
ISBN (Electronic)9780979806476
Publication statusPublished - 2014 Jan 1
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14/10/2614/10/30

Keywords

  • Fluorescence polarization
  • Fluorescence polarization immunoassay
  • Liquid-crystal display

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Wakao, O., Fujii, Y., Ishida, A., Tani, H., Hibara, A., & Tokeshi, M. (2014). LCD-CCD synchronization detection for fluorescence polarization immunoassay. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 2271-2273). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.