LC-MS/MS analysis of carboxymethylated and carboxyethylated phosphatidylethanolamines in human erythrocytes and blood plasma

Naoki Shoji, Kiyotaka Nakagawa, Akira Asai, Ikuko Fujita, Aya Hashiura, Yasushi Nakajima, Shinichi Oikawa, Teruo Miyazawa

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

An amino group of phosphatidylethanolamine (PE) is considered as a target for nonenzymatic glycation, and the potential involvement of lipid glycation in the pathogenesis of diabetic complications has generated interest. However, unlike an early glycation product of PE (Amadori-PE), the occurrence and roles of advanced glycation end products of PE (AGE-PE) in vivo have been unclear. Here, we developed an LC-MS/MS method for the analysis of AGE-PE [carboxymethyl-PE (CM-PE) and carboxyethyl-PE (CE-PE)]. Collision-induced dissociation of CM-PE and CE-PE produced characteristic ions, permitting neutral loss scanning (NLS) and multiple reaction monitoring (MRM) of AGE-PE. By NLS analysis, a series of AGE-PE molecular species was detected in human erythrocytes and blood plasma. In LC-MS/MS analysis, MRM enabled the separation and determination of the predominant AGE-PE species. Between healthy subjects and diabetic patients, no significant differences were observed in AGE-PE concentrations in erythrocytes and plasma, whereas Amadori-PE concentrations were higher in diabetic patients. These results provide direct evidence for the presence of AGE-PE in human blood, and indicated that, compared with Amadori-PE, AGE-PE is less likely to be accumulated in diabetic blood.jlr The presently developed LC-MS/MS method appears to be a powerful tool for understanding in vivo lipid glycation and its pathophysiological consequence.

Original languageEnglish
Pages (from-to)2445-2453
Number of pages9
JournalJournal of lipid research
Volume51
Issue number8
DOIs
Publication statusPublished - 2010 Aug

Keywords

  • Advanced glycation end products
  • Diabetes
  • Lipid glycation
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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