Layer-by-layer films composed of poly(allylamine) and insulin for pH-triggered release of insulin

Kentaro Yoshida, Ryosuke Hashide, Tsuyoshi Ishii, Shigehiro Takahashi, Katsuhiko Sato, Jun ichi Anzai

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Layer-by-layer (LbL) thin films containing insulin were prepared by alternately depositing insulin and poly(allylamine hydrochloride) (PAH) onto a solid surface at pH 7.4. The deposition behavior of the LbL films was studied using a quartz crystal microbalance, UV-vis absorption spectrometer, ζ-potential analyzer, and an atomic force microscope. The insulin-containing LbL films were stable in neutral pH media, but the films decomposed in solutions at pH 5.0 or lower. The decomposition of the LbL films can be rationalized on the basis of the loss of electrostatic interactions between insulin and PAH due to a shift in the net electric charge of insulin from negative to positive in acidic media. The films also decomposed to some extent in media at pH 9.0 and 10. A circular dichroism analysis showed that insulin retained its original conformation when released from LbL film into acidic solutions.

Original languageEnglish
Pages (from-to)274-279
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume91
Issue number1
DOIs
Publication statusPublished - 2012 Mar 1

Keywords

  • Drug delivery
  • Insulin
  • Layer-by-layer film
  • PH-response
  • Poly(allylamine)

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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