Layer-by-layer construction of protein architectures through avidin-biotin and lectin-sugar interactions for biosensor applications

Shigehiro Takahashi, Katsuhiko Sato, Jun Ichi Anzai

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

In this review, the preparation and properties of protein architectures constructed by layer-by-layer (LbL) deposition through avidin-biotin and concanavalin A (Con A) -sugar interactions are discussed in relation to their use for optical and electrochemical biosensors. LbL films can be constructed through the alternate deposition of avidin and biotin-labeled enzymes on the surfaces of optical probes and electrodes. The enzymes retain their catalytic activity, resulting in the formation of optical and electrochemical biosensors. Alternatively, Con A can be used to construct enzymecontaining LbL films and microcapsules using sugar-labeled enzymes. Some enzymes such as glucose oxidase and horseradish peroxidase can be used for this purpose without labeling with sugar, because these enzymes contain intrinsic hydrocarbon chains on their molecular surfaces. The Con A/enzyme LbL architectures were successfully used to develop biosensors sensitive to specific substrates of the enzyme. In addition, Con A-based films can be used for the optical and electrochemical detection of sugars.

Original languageEnglish
Pages (from-to)1749-1758
Number of pages10
JournalAnalytical and Bioanalytical Chemistry
Volume402
Issue number5
DOIs
Publication statusPublished - 2012 Feb 1

Keywords

  • Avidin-biotin
  • Biosensor
  • Layer-by-layer film
  • Lectin-sugar
  • Microcapsule
  • Protein architecture

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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